| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,810,989 | A→G | F135S (TTC→TCC) | chbG ← | chitin disaccharide deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,810,989 | 0 | A | G | 95.7% | 69.7 / ‑3.0 | 23 | F135S (TTC→TCC) | chbG | chitin disaccharide deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (11/11); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTC > NZ_CP009273/1810855‑1811122 | cACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAAt > 1:326812/1‑139 (MQ=255) aCTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGTTCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAAt < 2:35508/138‑1 (MQ=255) gTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGc < 2:73473/139‑1 (MQ=255) tAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACata > 1:233313/1‑135 (MQ=255) tAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACata < 2:233313/135‑1 (MQ=255) tAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGca > 2:288656/1‑139 (MQ=255) tAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGca > 2:284932/1‑139 (MQ=255) tAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGca < 2:360516/139‑1 (MQ=255) gAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGcac < 1:288656/138‑1 (MQ=255) aCTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatga > 1:104746/1‑139 (MQ=255) gggTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATg > 1:8393/1‑139 (MQ=255) gggTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATg > 2:208852/1‑139 (MQ=255) tGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAgg > 1:244963/1‑139 (MQ=255) aGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGc < 2:104746/139‑1 (MQ=255) gACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTcc > 2:237315/1‑138 (MQ=255) gACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTcc > 1:439321/1‑138 (MQ=255) aCCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCa < 1:111787/138‑1 (MQ=255) ccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACa < 2:8393/139‑1 (MQ=255) tctGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATgg > 2:262374/1‑60 (MQ=255) tctGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATgg < 1:262374/60‑1 (MQ=255) ccGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATttcttc < 2:439321/136‑1 (MQ=255) ccGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAg > 2:152021/1‑139 (MQ=255) tCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtcttcttc < 1:237315/139‑1 (MQ=255) | CACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTC > NZ_CP009273/1810855‑1811122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |