Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,856,434	A→G	S85P (TCA→CCA)	msrB ←	peptide‑methionine (R)‑S‑oxide reductase MsrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,856,434	0	A	G	100.0%	47.4 / NA	14	S85P (TCA→CCA)	msrB	peptide‑methionine (R)‑S‑oxide reductase MsrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/6);  total (8/6)

AAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATA  >  NZ_CP009273/1856317‑1856559                                                                                                                      |                                                                                                                              aaGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAAt                                                                                                          <  2:9903/139‑1 (MQ=255)        agagTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGAttct                                                                                                   <  2:217421/139‑1 (MQ=255)                    aTAACGTTCGCCCGTTGGCGGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGt                                                                                       >  1:367458/1‑139 (MQ=255)                           tCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGAt                                                                                                      <  1:55595/117‑1 (MQ=255)                           tCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGAt                                                                                                      >  2:55595/1‑117 (MQ=255)                             gCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGaaa                                                                              >  2:112322/1‑139 (MQ=255)                                                          cATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACtt                                                 <  1:10058/139‑1 (MQ=255)                                                          cATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACtt                                                 >  2:45661/1‑139 (MQ=255)                                                              ccccAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGtt                                             >  2:21530/1‑139 (MQ=255)                                                                       ggCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATg                                     >  2:197255/1‑138 (MQ=255)                                                                         cATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGaaa                                  >  1:431520/1‑139 (MQ=255)                                                                             acaGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAg                               <  2:429732/138‑1 (MQ=255)                                                                                                tCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCaaa            <  1:1230/138‑1 (MQ=255)                                                                                                         tGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGAtata  >  2:320863/1‑139 (MQ=255)                                                                                                                      |                                                                                                                              AAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATA  >  NZ_CP009273/1856317‑1856559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑