| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,879,784 | T→G | V288G (GTG→GGG) | yeaW → | carnitine monooxygenase subunit YeaW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,879,784 | 0 | T | G | 96.3% | 87.7 / ‑4.6 | 27 | V288G (GTG→GGG) | yeaW | carnitine monooxygenase subunit YeaW |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (18/8); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGT > NZ_CP009273/1879657‑1879907 | tCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGaaa > 1:364860/1‑139 (MQ=255) gTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTg < 1:8493/139‑1 (MQ=255) ggTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACt > 2:264454/1‑139 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACt < 2:71879/117‑1 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACt > 1:71879/1‑117 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg > 1:189842/1‑138 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg > 1:377940/1‑138 (MQ=255) gCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAag < 1:216541/139‑1 (MQ=255) tttCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcgac > 2:379068/1‑139 (MQ=255) tCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcgacg < 1:287104/138‑1 (MQ=255) tgGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg > 2:302864/1‑115 (MQ=255) tgGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg < 1:302864/115‑1 (MQ=255) tgGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAAt > 1:107231/1‑139 (MQ=255) tgGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAAt > 1:345450/1‑139 (MQ=255) gCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCg > 2:196554/1‑139 (MQ=255) aTGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt > 1:118571/1‑139 (MQ=255) aaCGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATc < 2:189842/139‑1 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 1:164321/1‑139 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 1:69207/1‑139 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 1:231547/1‑139 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 2:186518/1‑139 (MQ=255) aTCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg > 2:97291/1‑85 (MQ=255) aTCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg < 1:97291/85‑1 (MQ=255) aTCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAg > 1:415704/1‑120 (MQ=255) aTCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAg < 2:415704/120‑1 (MQ=255) tgatgaCGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCgg > 1:193181/1‑135 (MQ=255) tttATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGt > 1:240629/1‑139 (MQ=255) | TCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGT > NZ_CP009273/1879657‑1879907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |