Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,932,990	A→G	E362G (GAA→GGA)	pyk →	pyruvate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,932,990	0	A	G	100.0%	31.1 / NA	10	E362G (GAA→GGA)	pyk	pyruvate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/6);  total (4/6)

CGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCAT  >  NZ_CP009273/1932871‑1933125                                                                                                                        |                                                                                                                                        cGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCa                                                                                                                      <  1:371223/139‑1 (MQ=255)         aGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTa                                                                                                              <  2:392515/139‑1 (MQ=255)         aGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTa                                                                                                              <  2:75466/139‑1 (MQ=255)         aGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCACGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTa                                                                                                              <  2:73117/139‑1 (MQ=255)                gTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCt                                                                                                       <  2:279501/139‑1 (MQ=255)                     aaaCCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAacca                                                                                                  >  1:96177/1‑139 (MQ=255)                                       cgcgcgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGc                                                                                <  2:96177/139‑1 (MQ=255)                                                                      gAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTAcc                                                 >  2:140988/1‑139 (MQ=255)                                                                                            cGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTAt                             >  1:110998/1‑137 (MQ=255)                                                                                                                     tgGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCAt  >  2:312328/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                        CGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCAT  >  NZ_CP009273/1932871‑1933125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑