Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	243,919	A→T	R241W (AGG→TGG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	243,919	0	A	T	100.0%	82.1 / NA	21	R241W (AGG→TGG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/9);  total (12/9)

CGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGC  >  NZ_CP009273/243789‑244053                                                                                                                                   |                                                                                                                                       cgAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGAtttt                                                                                                                                 >  1:201146/1‑138 (MQ=255) cgAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGAtttt                                                                                                                                 >  1:587/1‑138 (MQ=255)           gATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAgaga                                                                                                                      <  1:194487/139‑1 (MQ=255)                      cGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAg                                                                                                           >  2:139499/1‑139 (MQ=255)                      cGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAg                                                                                                           >  1:295337/1‑139 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                         >  2:166812/1‑139 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCCAGTCGCCGCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTATGa                                                                                                         <  2:80828/139‑1 (MQ=255)                          tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACg                                                                                                       >  2:199695/1‑139 (MQ=255)                               gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGAt                                                                                                    >  2:260152/1‑137 (MQ=255)                               gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGAt                                                                                                    >  2:404686/1‑137 (MQ=255)                                         cAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTAcg                                                                                        <  1:166812/139‑1 (MQ=255)                                                       gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                           <  2:201146/138‑1 (MQ=255)                                                                     aaaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                            <  1:366680/139‑1 (MQ=255)                                                                            tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                      >  2:428313/1‑138 (MQ=255)                                                                                     ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                            >  1:173676/1‑139 (MQ=255)                                                                                            gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                     <  1:139499/139‑1 (MQ=255)                                                                                                             ggACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcg                    >  2:404615/1‑139 (MQ=255)                                                                                                                              cgcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                              <  1:249276/64‑1 (MQ=255)                                                                                                                              cgcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                              >  2:249276/1‑64 (MQ=255)                                                                                                                              cgcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg   <  1:404686/139‑1 (MQ=255)                                                                                                                               gcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc  <  1:404615/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGC  >  NZ_CP009273/243789‑244053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑