Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,941,091	A→G	E195G (GAA→GGA)	yebB →	YebB family permuted papain‑like enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,941,091	0	A	G	100.0%	29.6 / NA	10	E195G (GAA→GGA)	yebB	YebB family permuted papain‑like enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/8);  total (2/8)

ACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCA  >  NZ_CP009273/1940964‑1941183                                                                                                                                |                                                                                             aCTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAgg                                                                                   <  1:106792/139‑1 (MQ=255) aCTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAg                                                                                    <  1:313936/138‑1 (MQ=255)                                ttCCGTGGGAGCGTAAAGCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGcc                                                    <  1:380876/139‑1 (MQ=255)                                ttCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGcc                                                    <  1:52879/139‑1 (MQ=255)                                                  ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCg                                  <  2:50751/139‑1 (MQ=255)                                                                        gCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGAc            <  2:384034/139‑1 (MQ=255)                                                                            catcCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc        >  1:404217/1‑139 (MQ=255)                                                                             attcGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCTGGACTGACCGAGGCGGTATAACTTAACGCAGTCGTCCTCTCGCCCGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTacc        <  2:247914/135‑3 (MQ=255)                                                                                        gtgtTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCTCTCATCTGCATCGCATTCTGACTAACGTGGCa  <  1:387603/133‑1 (MQ=255)                                                                                        gtgtTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCa  >  2:387603/1‑133 (MQ=255)                                                                                                                                |                                                                                             ACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCA  >  NZ_CP009273/1940964‑1941183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑