Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,955,016	A→G	D233G (GAC→GGC)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,955,016	0	A	G	100.0%	49.8 / NA	15	D233G (GAC→GGC)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/10);  total (5/10)

GGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATG  >  NZ_CP009273/1954889‑1955110                                                                                                                                |                                                                                               ggTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGa                                                                                     <  2:162262/139‑1 (MQ=255)             gATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGa                                                                         <  1:64068/139‑1 (MQ=255)             gATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGa                                                                         <  2:27883/139‑1 (MQ=255)                       aGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                               <  2:130398/139‑1 (MQ=255)                           nnaNGNTGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGt                                                           <  2:367101/137‑1 (MQ=255)                                                cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                      >  2:159410/1‑139 (MQ=255)                                                cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                      >  2:248152/1‑139 (MQ=255)                                                                 aCTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCg                      <  1:248152/138‑1 (MQ=255)                                                                          tAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTa            <  2:326507/139‑1 (MQ=255)                                                                                tGCAAAGCGGTGACGAATATTTCCACGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAAccc      >  1:273792/1‑139 (MQ=255)                                                                                  cAAAGCGGTGATGAATATTGTCGAGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATTGTATCAATGTGACGTTGAAAAGTGATGACGTGATGGGCGAAAGCCTATATaag        >  1:303077/1‑134 (MQ=255)                                                                                    aaGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGAtg  <  1:159410/139‑1 (MQ=255)                                                                                    aaGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGAtg  <  2:274938/139‑1 (MQ=255)                                                                                                           agaTGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGa    >  1:276376/1‑114 (MQ=255)                                                                                                           agaTGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGa    <  2:276376/114‑1 (MQ=255)                                                                                                                                |                                                                                               GGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATG  >  NZ_CP009273/1954889‑1955110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑