| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,000,880 | A→G | D415G (GAC→GGC) | amyA → | alpha‑amylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,000,880 | 0 | A | G | 100.0% | 53.7 / NA | 16 | D415G (GAC→GGC) | amyA | alpha‑amylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/7); total (9/7) | |||||||||||
CGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCT > NZ_CP009273/2000754‑2001008 | cgcATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTg > 2:217530/1‑139 (MQ=255) tCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGcc < 2:77424/138‑1 (MQ=255) aCGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAc > 2:223678/1‑139 (MQ=255) gCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACcgac > 2:259687/1‑139 (MQ=255) aaCAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGaa > 1:168595/1‑139 (MQ=255) aaCAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGaa > 1:71163/1‑139 (MQ=255) gatgaGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGgat < 1:217530/138‑1 (MQ=255) cGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCg < 1:409386/101‑1 (MQ=255) cGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCg > 2:409386/1‑101 (MQ=255) cccGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCAt < 2:71163/139‑1 (MQ=255) cGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTAc < 1:223678/139‑1 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCg < 1:183095/81‑1 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCg > 2:183095/1‑81 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCt > 1:282319/1‑139 (MQ=255) aTTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCg > 1:26962/1‑95 (MQ=255) aTTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCg < 2:26962/95‑1 (MQ=255) | CGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCT > NZ_CP009273/2000754‑2001008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |