Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,037,050	T→G	intergenic (‑12/‑82)	BW25113_RS10320 ← / → mtfA	tRNA‑Ser/DgsA anti‑repressor MtfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,037,050	0	T	G	100.0%	48.9 / NA	14	intergenic (‑12/‑82)	BW25113_RS10320/mtfA	tRNA‑Ser/DgsA anti‑repressor MtfA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/8);  total (6/8)

TGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACA  >  NZ_CP009273/2036941‑2037157                                                                                                              |                                                                                                            tGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCAtttt                                                                                 >  1:418668/1‑138 (MQ=255)   aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                               <  1:25427/138‑1 (MQ=255)   aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                               <  2:351954/138‑1 (MQ=255)   aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                               <  2:364300/138‑1 (MQ=255)                                           gTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGc                                      >  2:39906/1‑139 (MQ=255)                                             tGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGc                                      >  1:970/1‑137 (MQ=255)                                                               cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                   <  1:39906/138‑1 (MQ=255)                                                               cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                   <  2:250191/138‑1 (MQ=255)                                                                            ttCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACg                            <  1:69691/116‑1 (MQ=255)                                                                            ttCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACg                            >  2:69691/1‑116 (MQ=255)                                                                               aGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACa  <  2:295888/139‑1 (MQ=255)                                                                                 ccGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTAc   >  2:211988/1‑136 (MQ=255)                                                                                    cTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACa  >  1:375324/1‑134 (MQ=255)                                                                                    cTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACa  <  2:375324/134‑1 (MQ=255)                                                                                                              |                                                                                                            TGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACA  >  NZ_CP009273/2036941‑2037157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑