| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,039,724 | A→G | T436A (ACA→GCA) | yeeJ → | inverse autotransporter adhesin YeeJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,039,724 | 0 | A | G | 95.7% | 76.5 / ‑4.3 | 23 | T436A (ACA→GCA) | yeeJ | inverse autotransporter adhesin YeeJ |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (13/9); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CGCAATGCAGAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGT > NZ_CP009273/2039588‑2039848 | cgcAATGCAGAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCa < 2:423577/139‑1 (MQ=255) cTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAgg > 1:117540/1‑137 (MQ=255) cTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAgg < 2:117540/137‑1 (MQ=255) gAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGTAGCCGTTATGATCTGGTGGATCGCACCAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGga < 1:197866/139‑1 (MQ=255) tGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTa > 2:440228/1‑139 (MQ=255) caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc > 1:349355/1‑138 (MQ=255) caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc > 2:434074/1‑138 (MQ=255) agCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAAcc > 1:421875/1‑138 (MQ=255) gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTg < 1:187751/135‑1 (MQ=255) gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTg < 1:174178/135‑1 (MQ=255) gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAg > 1:17948/1‑139 (MQ=255) gCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtata < 2:349355/138‑1 (MQ=255) tGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCa < 2:438979/41‑1 (MQ=255) tGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCa > 1:438979/1‑41 (MQ=255) gAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTg > 2:226666/1‑138 (MQ=255) tCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtata < 1:183418/113‑1 (MQ=255) tCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtata > 2:183418/1‑113 (MQ=255) tCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCt > 1:178780/1‑139 (MQ=255) tCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCt > 1:272625/1‑139 (MQ=255) cGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTg > 2:226664/1‑139 (MQ=255) aCTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCaaa < 1:440228/139‑1 (MQ=255) aCTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCaaa < 2:74493/139‑1 (MQ=255) tCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGt > 1:157516/1‑138 (MQ=255) | CGCAATGCAGAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGT > NZ_CP009273/2039588‑2039848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |