Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,069,946	A→G	Y53C (TAT→TGT)	yeeS →	RadC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,069,946	0	A	G	100.0%	43.2 / NA	14	Y53C (TAT→TGT)	yeeS	RadC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/7);  total (7/7)

TTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGTGGTACTGGC  >  NZ_CP009273/2069814‑2070075                                                                                                                                     |                                                                                                                                  ttCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGa                                                                                                                              >  1:185543/1‑138 (MQ=255)    aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGaacaa                                                                                                                          <  2:185543/139‑1 (MQ=255)     aCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGaacaac                                                                                                                         <  2:303673/139‑1 (MQ=255)            cTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAAt                                                                                                                   >  1:44113/1‑138 (MQ=255)                                                                    tGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCAt                                                          >  2:195760/1‑139 (MQ=255)                                                                                   gCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGAt                                            >  2:318266/1‑138 (MQ=255)                                                                                      gATTCTGAACATGGCGGGAATGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGGATCAGCTGATTGCCGGTGAAAACCTCTTCACCGGCACCATCAACCACACGGAAATACATCCACAGGGAGGGATTaa                                         >  2:130246/1‑138 (MQ=255)                                                                                             aaCATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAcgcg                                    <  1:194819/136‑1 (MQ=255)                                                                                             aaCATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGcc                                  <  2:151441/138‑1 (MQ=255)                                                                                                  ggCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTAc                            >  2:126194/1‑139 (MQ=255)                                                                                                  ggCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTAc                            >  2:321773/1‑139 (MQ=255)                                                                                                              gCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCt                <  1:321773/139‑1 (MQ=255)                                                                                                                gTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGc              <  2:321118/139‑1 (MQ=255)                                                                                                                            gggTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGTGGTACTGGc  <  1:121722/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  TTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGTGGTACTGGC  >  NZ_CP009273/2069814‑2070075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑