Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,088,811	T→C	V69A (GTC→GCC)	hisA →	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,088,811	0	T	C	100.0%	47.3 / NA	14	V69A (GTC→GCC)	hisA	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

AGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCG  >  NZ_CP009273/2088676‑2088900                                                                                                                                        |                                                                                          aGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCcgc                                                                                        <  1:292770/139‑1 (MQ=255)                        cTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAgg                                                                 >  2:45317/1‑139 (MQ=255)                                    cAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTggcggcggc                                                      <  1:247125/138‑1 (MQ=255)                                                 cgcAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCgaag                                         >  1:317066/1‑138 (MQ=255)                                                          ccGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTggc                               <  2:438670/139‑1 (MQ=255)                                                                  ctgcACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTggcgg                             >  1:350233/1‑133 (MQ=255)                                                                  ctgcACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTggcgg                             <  2:350233/133‑1 (MQ=255)                                                                          ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGt                                                                <  2:383633/90‑1 (MQ=255)                                                                          ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCg               >  1:3039/1‑139 (MQ=255)                                                                          ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCg               >  2:71474/1‑139 (MQ=255)                                                                          ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCg               >  2:86925/1‑139 (MQ=255)                                                                          ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCAGGTGCAGGt                                                                >  1:383633/1‑90 (MQ=255)                                                                            tggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCggg             >  1:38947/1‑139 (MQ=255)                                                                                       ggggCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGcgcgcg  <  2:350334/139‑1 (MQ=255)                                                                                                                                        |                                                                                          AGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCG  >  NZ_CP009273/2088676‑2088900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑