Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,135,155	T→C	N181S (AAC→AGC)	dcd ←	dCTP deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,135,155	0	T	C	89.5%	47.5 / 0.5	19	N181S (AAC→AGC)	dcd	dCTP deaminase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (9/8);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

CTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGT  >  NZ_CP009273/2135027‑2135275                                                                                                                                 |                                                                                                                         cTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATAttt                                                                                                                >  1:262926/1‑139 (MQ=255)          aTAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCtt                                                                                                        >  1:273979/1‑138 (MQ=255)            aaCCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCAc                                                                                                     >  1:325389/1‑139 (MQ=255)              cccGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCAcgg                                                                                                   <  2:262926/139‑1 (MQ=255)               ccGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCAcggc                                                                                                  <  2:352477/139‑1 (MQ=255)                            cGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCg                                                                                     <  2:273979/139‑1 (MQ=255)                                    aGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGGAAGGGCGCAccgccg                                                                             >  1:292738/1‑139 (MQ=255)                                           tcaGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGGCGCACCcccggggcgg                                                                       >  2:290455/1‑138 (MQ=255)                                                                  aTGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAgcg                                                  <  2:325389/136‑1 (MQ=255)                                                                             aaTGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGGCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATg                                    >  2:373091/1‑139 (MQ=255)                                                                                    cTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCa                                                            >  1:248780/1‑108 (MQ=255)                                                                                    cTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCa                                                            <  2:248780/108‑1 (MQ=255)                                                                                       aaTTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACg                            <  2:425789/137‑1 (MQ=255)                                                                                       aaTTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACg                            <  2:437758/137‑1 (MQ=255)                                                                                           aGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCCCCAATTAACATGCCCGGGCGCAgcgc                      >  1:168616/1‑139 (MQ=255)                                                                                            gTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGGCGCACCGCCGGGCCGGGAAGCGGCTCAAAGCTCCGCGCACCAATTAACATGCCCCGCCGCAGCGcc                     >  1:373483/1‑139 (MQ=255)                                                                                                 tATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGa                              <  2:61919/125‑1 (MQ=255)                                                                                                 tATCGATTCGGCTGGCTACCGCGCCCTGCTGGCTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAAAATGCCCGGa                              >  1:61919/1‑125 (MQ=255)                                                                                                               gctACCGCGCCCTGCTGGTTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGt  <  1:290455/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                         CTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATTTCGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGT  >  NZ_CP009273/2135027‑2135275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑