Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,179,484	A→G	E30G (GAA→GGA)	rcnA →	nickel/cobalt efflux protein RcnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,179,484	0	A	G	100.0%	66.4 / NA	20	E30G (GAA→GGA)	rcnA	nickel/cobalt efflux protein RcnA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/8);  total (12/8)

GGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATT  >  NZ_CP009273/2179349‑2179605                                                                                                                                        |                                                                                                                          ggggggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGAc                                                                                                                         >  1:106175/1‑138 (MQ=255) ggggggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGAc                                                                                                                         >  1:50069/1‑138 (MQ=255)              tCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTAGTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCaaa                                                                                                            >  1:344412/1‑138 (MQ=255)                                aCTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGtt                                                                                          >  1:3526/1‑138 (MQ=255)                                    aGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTatca                                                                                     >  2:294502/1‑139 (MQ=255)                                          attatCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGcc                                                                                <  2:3526/138‑1 (MQ=255)                                                aTGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGAGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCACAg                                                                         >  2:316036/1‑139 (MQ=255)                                                   aCCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCa                                                                      >  2:188844/1‑139 (MQ=255)                                                       aaTTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCAt                                                                  >  1:348784/1‑139 (MQ=255)                                                            aCAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTaaa                                                              >  2:403896/1‑138 (MQ=255)                                                                     cttcAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAgg                                                                        <  1:234280/119‑1 (MQ=255)                                                                     cttcAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAgg                                                                        >  2:234280/1‑119 (MQ=255)                                                                     cttcAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCgg                                                       <  1:140848/136‑1 (MQ=255)                                                                     cttcAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGt                                                      <  2:117310/137‑1 (MQ=255)                                                                                  aCGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGgc                                       <  1:201226/139‑1 (MQ=255)                                                                                  aCGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGgc                                       <  1:188844/139‑1 (MQ=255)                                                                                                        cgcCATCTTACTTGGTGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCa                 <  2:183198/139‑1 (MQ=255)                                                                                                                       tGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTg                                                     >  2:259315/1‑88 (MQ=255)                                                                                                                       tGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTg                                                     <  1:259315/88‑1 (MQ=255)                                                                                                                       tGCGCTTCATGGCCTGGGACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAAtt  >  2:340701/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                          GGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAAGCGGTGATGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATT  >  NZ_CP009273/2179349‑2179605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑