Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,258,402	T→A	I354N (ATC→AAC)	setB →	sugar efflux transporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,258,402	0	T	A	100.0%	95.7 / NA	25	I354N (ATC→AAC)	setB	sugar efflux transporter SetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/12);  total (13/12)

GCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATTATC‑GCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGC  >  NZ_CP009273/2258270‑2258535                                                                                                                                     |                                                                                                                                       gCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGc                                                                                                                                  <  1:268063/139‑1 (MQ=255)     gCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGa                                                                                                                              >  2:392304/1‑139 (MQ=255)      cAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGAt                                                                                                                             <  1:220577/139‑1 (MQ=255)          tgctAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGt                                                                                                                         >  2:2409/1‑139 (MQ=255)                      tttttATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCAtcgt                                                                                                             >  2:313171/1‑139 (MQ=255)                           aTTGGCATTCTGGGCGGCATCGTGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGcc                                                                                                         <  1:210327/138‑1 (MQ=255)                           aTTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGcc                                                                                                         <  1:392304/138‑1 (MQ=255)                                             aTCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                                                                                      <  1:346509/139‑1 (MQ=255)                                             aTCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                                                                                      <  1:170208/139‑1 (MQ=255)                                                gggATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCt                                                                                    <  2:253067/138‑1 (MQ=255)                                                                        aTGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGt                                                                <  1:2409/134‑1 (MQ=255)                                                                               gTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATTGTCGCCCAGGTCTGGGATTATTCCGCTGTGTTTTTGTTTGCGGTGGGGATGGTTATC‑Gc                                                   >  1:378118/1‑139 (MQ=255)                                                                                                           taCCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATTATC‑GCCACTCTGTTTTGCTTACTGCGGATTaaa                       <  1:313171/139‑1 (MQ=255)                                                                                                            aCCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGGCGCCGGGGTCTGGAATTATCACGCCGTGTTCTGGGTTGCGATGGTGGTGGTTATT‑GCCACTCTGTTTTGCTTATTGCGGATTa                         >  2:102265/1‑136 (MQ=255)                                                                                                                 cacTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGGGATCTGGGATTATCACGCTGTGTTCTGGTTTGCGATGGGGGTGATTATC‑GCCCCTCTGGTTTTCTTACTGCGGGTTAAAGATggt                 >  1:245086/1‑137 (MQ=255)                                                                                                                 cacTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGCGATCTGGGATTATCACGCTGTGTTCTGGTTTGCGCTGGGGGTGATTTTT‑GCCCCTCTGTTTTTCTTTCTGCGGGTTAAAGATGtt                 >  2:203392/1‑139 (MQ=255)                                                                                                                 cacTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGGTCTGGGATTATCACGCTGTGTTCTGGGTTTCGGTGGGGGTGATTATC‑GCCACTCTGGTTTGCTTACTGGGGGTTAAAGaaggt                 >  2:61676/1‑135 (MQ=255)                                                                                                                   cTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGGTCTGGAAATATCACGCTGTGGTCTGGGTTGCGGTTGTGGTGGTTATC‑GCCACTCTGTTTTGCTTACAGCGGATTACAGATGgtt                >  1:133445/1‑138 (MQ=255)                                                                                                                   cTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCt                                                                                    >  1:274523/1‑71 (MQ=255)                                                                                                                   cTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCt                                                                                    <  2:274523/71‑1 (MQ=255)                                                                                                                   cTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATTATC‑GCCACTTTGTTTTTCTTACTGCGGGTTAAAGATGttt                >  1:77881/1‑138 (MQ=255)                                                                                                                     tCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGGATCCCCGCCCGGGACTGGAATTAGTCGGCTGCGTTCTGGGTTTTGTCGGAGAAGATTTTCGGCCCCTC‑GTTTTGGTCATTGCGGTTTTAAGTCGTTTa               >  1:163699/1‑137 (MQ=255)                                                                                                                     tCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGGATTATCACGCTGTGTTCTGGGTTGCGATGGTGGTGATTATC‑GCCACTCTGTTTTTCTTACTGCGGGTTAAAGATGTTTTAg             >  2:180161/1‑139 (MQ=255)                                                                                                                             gggCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATTATC‑GCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTc     <  1:203392/139‑1 (MQ=255)                                                                                                                                cTGGAACATCGCAGGATCAGTGGCGGGTATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATTATC‑GCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGc  <  2:83733/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATTATC‑GCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGC  >  NZ_CP009273/2258270‑2258535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑