| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,268,854 | A→G | S35G (AGT→GGT) | yejE → | ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,268,854 | 0 | A | G | 94.7% | 56.1 / ‑3.3 | 19 | S35G (AGT→GGT) | yejE | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (6/12); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTG > NZ_CP009273/2268720‑2268967 | gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt > 1:205107/1‑139 (MQ=255)gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt > 1:288372/1‑139 (MQ=255)gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt < 2:240682/139‑1 (MQ=255) gcgTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTg < 2:205107/139‑1 (MQ=255) ttGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGc < 2:278463/138‑1 (MQ=255) gAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCa < 1:413662/138‑1 (MQ=255) aTGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGt > 2:177339/1‑139 (MQ=255) cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCaa > 1:239526/1‑122 (MQ=255) cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCaa < 2:239526/122‑1 (MQ=255) gACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGt < 1:227181/139‑1 (MQ=255) tCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCa < 2:99066/108‑1 (MQ=255) tCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCa > 1:99066/1‑108 (MQ=255) aGGCCCGTTGGTCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTg < 1:357024/139‑1 (MQ=255) cgcgGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTg < 2:184433/58‑1 (MQ=255) cgcgGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTg > 1:184433/1‑58 (MQ=255) gcgGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGaaa < 1:368541/138‑1 (MQ=255) ttATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGcc > 1:185357/1‑139 (MQ=255) aTGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGccc < 1:349164/138‑1 (MQ=255) gATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTg < 2:185357/139‑1 (MQ=255) | GTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTG > NZ_CP009273/2268720‑2268967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |