| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,287,796 | C→T | L196L (CTG→CTA) | ccmF ← | cytochrome c‑type biogenesis heme lyase CcmF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,287,796 | 0 | C | T | 87.0% | 67.4 / 1.5 | 23 | L196L (CTG→CTA) | ccmF | cytochrome c‑type biogenesis heme lyase CcmF |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (12/8); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCG > NZ_CP009273/2287667‑2287920 | ccaGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAaag > 2:71948/1‑137 (MQ=255) aGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAaagaag > 2:190808/1‑138 (MQ=255) cccAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATgg > 1:55589/1‑138 (MQ=255) cccAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGc < 1:190808/139‑1 (MQ=255) gCCCATGCGGAACCAAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCgag > 1:235552/1‑138 (MQ=255) cGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCATCATAGAATCAATGGCAAAAGCGAACGCCACCGAGAAACCCAc < 2:155027/139‑1 (MQ=255) ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGTAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc < 2:340007/139‑1 (MQ=255) ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc < 2:429989/139‑1 (MQ=255) gCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc > 2:362968/1‑106 (MQ=255) gCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc < 1:362968/106‑1 (MQ=255) cTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAACCGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGccc > 2:316455/1‑139 (MQ=255) gACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAg < 2:116329/139‑1 (MQ=255) gTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGt > 2:72533/1‑139 (MQ=255) tAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGt > 1:298608/1‑138 (MQ=255) aaaCGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTa < 2:55589/138‑1 (MQ=255) aaCGCGCATAAGTGCTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAg < 2:427221/139‑1 (MQ=255) cTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc > 2:30707/1‑67 (MQ=255) cTGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTAcc < 1:30707/67‑1 (MQ=255) tGTCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt > 2:89549/1‑139 (MQ=255) tCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt > 2:52981/1‑92 (MQ=255) tCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt < 1:52981/92‑1 (MQ=255) tCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCg > 2:356668/1‑139 (MQ=255) tCCAGACGCCCGCTTAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCg > 1:55039/1‑139 (MQ=255) | CCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCG > NZ_CP009273/2287667‑2287920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |