Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	251,113	A→G	I364T (ATC→ACC)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	251,113	0	A	G	100.0%	64.4 / NA	19	I364T (ATC→ACC)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

TCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGA  >  NZ_CP009273/250982‑251238                                                                                                                                    |                                                                                                                              tCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                          >  1:242437/1‑137 (MQ=255)              aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACg                                                                                                           >  2:395257/1‑139 (MQ=255)                  tCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTAt                                                                                                       >  1:243793/1‑139 (MQ=255)                                        cgcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACAcc                                                                                 >  2:393833/1‑139 (MQ=255)                                                    cGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAggg                                                                      <  2:16183/138‑1 (MQ=255)                                                    cGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAggg                                                                      <  2:225602/138‑1 (MQ=255)                                                                          aCCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGc                                                <  2:243793/138‑1 (MQ=255)                                                                            ccAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTa                                              >  1:397269/1‑138 (MQ=255)                                                                            ccAGCGAATCCAGCATACTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTa                                              >  1:418184/1‑138 (MQ=255)                                                                                             cTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca                            <  1:342525/139‑1 (MQ=255)                                                                                                accacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac                           <  2:242437/137‑1 (MQ=255)                                                                                                   acGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGt                      <  2:63017/139‑1 (MQ=255)                                                                                                    cGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGtt                     <  1:58043/139‑1 (MQ=255)                                                                                                    cGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGtt                     <  1:231532/139‑1 (MQ=255)                                                                                                       aGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCgg                  >  2:328258/1‑139 (MQ=255)                                                                                                                   tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGt                                                                                          >  2:278959/1‑55 (MQ=255)                                                                                                                   tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGt                                                                                          <  1:278959/55‑1 (MQ=255)                                                                                                                   tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcag      >  1:378071/1‑139 (MQ=255)                                                                                                                       gATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGa  <  2:53986/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                              TCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGA  >  NZ_CP009273/250982‑251238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑