Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,370,881	A→G	V64A (GTC→GCC)	menH ←	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,370,881	0	A	G	100.0%	42.6 / NA	13	V64A (GTC→GCC)	menH	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/4);  total (9/4)

GCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCG  >  NZ_CP009273/2370785‑2371009                                                                                                 |                                                                                                                                 gccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGt                                                                                        >  1:256389/1‑139 (MQ=255)               cACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCa                                                                          <  2:296200/139‑1 (MQ=255)                  ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt                                                                            >  1:155234/1‑134 (MQ=255)                  ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt                                                                            >  1:76521/1‑134 (MQ=255)                  ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt                                                                            <  2:155234/134‑1 (MQ=255)                  ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt                                                                            <  2:76521/134‑1 (MQ=255)                                                       aGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTgg                                                                                 <  1:191881/92‑1 (MQ=255)                                                       aGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTgg                                                                                 >  2:191881/1‑92 (MQ=255)                                                       aGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActt                                  >  1:98945/1‑139 (MQ=255)                                                                          aGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATc               >  1:360836/1‑139 (MQ=255)                                                                                tACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCgg          >  1:164393/1‑138 (MQ=255)                                                                                tACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCgg          >  2:336412/1‑138 (MQ=255)                                                                                       tAAGTCGGTGGCATCATCGAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCg  >  1:203434/1‑139 (MQ=255)                                                                                                 |                                                                                                                                 GCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCG  >  NZ_CP009273/2370785‑2371009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑