Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,406,008	A→G	D22D (GAT→GAC)	yfbU ←	YfbU family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,406,008	0	A	G	100.0%	153.7 / NA	43	D22D (GAT→GAC)	yfbU	YfbU family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATA  >  NZ_CP009273/2405878‑2406137                                                                                                                                   |                                                                                                                                  tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCa                                                                                                                            >  1:98242/1‑138 (MQ=255) tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCa                                                                                                                            >  1:33467/1‑138 (MQ=255)    tGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGt                                                                                                                        >  2:322411/1‑139 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      <  2:398383/139‑1 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      <  2:87896/139‑1 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      <  2:5981/139‑1 (MQ=255)        gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtcatc                                                                                                                    <  2:98242/139‑1 (MQ=255)         atgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      >  1:207575/1‑136 (MQ=255)         atgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      <  2:207575/136‑1 (MQ=255)           gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      <  2:6896/134‑1 (MQ=255)           gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      >  1:6896/1‑134 (MQ=255)                tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      <  1:265084/129‑1 (MQ=255)                tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                      >  2:265084/1‑129 (MQ=255)                         tttCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGa                                                                                                   <  2:33467/139‑1 (MQ=255)                         tttCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGa                                                                                                   <  2:374450/139‑1 (MQ=255)                                  tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAt                                                                                             >  1:118938/1‑136 (MQ=255)                                                    gATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAt                                                                        <  2:251375/139‑1 (MQ=255)                                                              aCGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgtt                                                              <  1:382937/139‑1 (MQ=255)                                                                       tAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTc                                                         <  2:6822/135‑1 (MQ=255)                                                                         aTCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt                                                   <  1:403777/139‑1 (MQ=255)                                                                         aTCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt                                                   <  2:341153/139‑1 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCa                                                 <  2:213485/138‑1 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                              >  1:177962/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                              >  2:261081/1‑139 (MQ=255)                                                                                 ccACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTctac                                           >  1:291444/1‑139 (MQ=255)                                                                                     gCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACg                                       >  2:422820/1‑139 (MQ=255)                                                                                      cTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTg                                                                             <  1:43939/100‑1 (MQ=255)                                                                                      cTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTg                                                                             >  2:43939/1‑100 (MQ=255)                                                                                         aataatTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTg                                    >  1:11130/1‑138 (MQ=255)                                                                                                 tttGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGaaaaaa                              >  1:238013/1‑136 (MQ=255)                                                                                                   tGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAaag                           >  2:114962/1‑137 (MQ=255)                                                                                                   tGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAaag                           >  1:63317/1‑137 (MQ=255)                                                                                                   tGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAaag                           >  1:387501/1‑137 (MQ=255)                                                                                                     cAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGa                                                            <  2:287429/102‑1 (MQ=255)                                                                                                     cAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGa                                                            >  1:287429/1‑102 (MQ=255)                                                                                                     cAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAgg                       >  1:140171/1‑139 (MQ=255)                                                                                                      aGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGc                      <  2:291444/139‑1 (MQ=255)                                                                                                      aGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGc                      <  1:114962/139‑1 (MQ=255)                                                                                                                  cGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCctt          >  1:257617/1‑139 (MQ=255)                                                                                                                    ttCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttct        >  1:161136/1‑139 (MQ=255)                                                                                                                         cATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtat   <  2:118938/139‑1 (MQ=255)                                                                                                                         cATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtat   <  2:63317/139‑1 (MQ=255)                                                                                                                          aTTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata  <  2:11130/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATA  >  NZ_CP009273/2405878‑2406137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑