Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,503,481	A→G	T125A (ACC→GCC)	yfeO →	ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,503,481	0	A	G	100.0%	59.2 / NA	17	T125A (ACC→GCC)	yfeO	ion channel protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/12);  total (5/12)

GACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCG  >  NZ_CP009273/2503353‑2503580                                                                                                                                 |                                                                                                    gACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAatat                                                                                           <  2:227435/139‑1 (MQ=255) ccccAGCCCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAatat                                                                                           <  2:309264/137‑1 (MQ=255)  aCCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATc                                                                                          >  2:296163/1‑139 (MQ=255)                   aaCCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCAt                                                                         <  1:296163/139‑1 (MQ=255)                        cTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcg                                                                    >  2:323590/1‑139 (MQ=255)                           aTCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                   <  2:300544/137‑1 (MQ=255)                           aTCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                   <  2:360723/137‑1 (MQ=255)                           aTCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                   <  2:418676/137‑1 (MQ=255)                           aTCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTc                                                                 <  2:321238/139‑1 (MQ=255)                                                             gACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                <  2:210250/138‑1 (MQ=255)                                                                   tCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                >  1:339782/1‑132 (MQ=255)                                                                   tCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                <  2:339782/132‑1 (MQ=255)                                                                   tCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTAttt                          >  1:348615/1‑138 (MQ=255)                                                                        gCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGcc                     <  1:323590/138‑1 (MQ=255)                                                                                         tGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGgc   >  1:166877/1‑139 (MQ=255)                                                                                           cTGGCGGCGTCAGCCTGGGGCCTGAACATCCTATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGgcg  <  1:440957/138‑1 (MQ=255)                                                                                           cTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGgcg  <  2:201113/138‑1 (MQ=255)                                                                                                                                 |                                                                                                    GACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCG  >  NZ_CP009273/2503353‑2503580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑