Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,521,647	C→T	G630D (GGC→GAC)	ligA ←	NAD‑dependent DNA ligase LigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,521,647	0	C	T	100.0%	101.4 / NA	26	G630D (GGC→GAC)	ligA	NAD‑dependent DNA ligase LigA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

TTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCG  >  NZ_CP009273/2521512‑2521782                                                                                                                                        |                                                                                                                                        tttCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCcg                                                                                                                                      >  1:74085/1‑139 (MQ=255)                   aGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGtt                                                                                                                    >  1:151709/1‑139 (MQ=255)                          gcagcaTTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAg                                                                                                             >  2:262634/1‑139 (MQ=255)                                           tcgATGACTTCAATGCCCAGTTCCTGTGCTCTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGTCCAGTCGAGCTTTAGCGtcat                                                                                            >  2:73074/1‑139 (MQ=255)                                           tcgATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGtcat                                                                                            >  2:149866/1‑139 (MQ=255)                                               tGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCAcg                                                                                        <  1:137624/139‑1 (MQ=255)                                                     cAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAg                                                                                                             <  1:373097/112‑1 (MQ=255)                                                     cAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAg                                                                                                             >  2:373097/1‑112 (MQ=255)                                                      aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  2:151709/138‑1 (MQ=255)                                                      aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                                  <  1:92974/138‑1 (MQ=255)                                                               ttCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAg                                                                        <  2:266448/139‑1 (MQ=255)                                                                  cTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTg                                                                     <  2:315832/139‑1 (MQ=255)                                                                    gcgcCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGcc                                                                   >  2:167395/1‑139 (MQ=255)                                                                           cGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAg                                                             <  2:400702/138‑1 (MQ=255)                                                                              cAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGcacc                                                         <  2:39705/139‑1 (MQ=255)                                                                                         cTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACAGTTTTAc                                               <  1:179058/138‑1 (MQ=255)                                                                                          tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTAc                                               >  1:90240/1‑137 (MQ=255)                                                                                           gCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAg                                            >  1:297849/1‑139 (MQ=255)                                                                                           gCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAg                                            >  2:375147/1‑139 (MQ=255)                                                                                                  cACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACgg                                     >  2:309371/1‑139 (MQ=255)                                                                                                   aCCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACggg                                    <  1:149866/139‑1 (MQ=255)                                                                                                    cccGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGc                                   <  1:74768/139‑1 (MQ=255)                                                                                                    cccGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTGCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGc                                   <  1:382319/139‑1 (MQ=255)                                                                                                                  aTCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTcc                     >  1:73700/1‑139 (MQ=255)                                                                                                                       ttttCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGtt                >  2:193217/1‑139 (MQ=255)                                                                                                                                     cTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGgcg  <  1:262634/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCG  >  NZ_CP009273/2521512‑2521782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑