Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,550,584	A→G	S25P (TCA→CCA)	eutC ←	ethanolamine ammonia‑lyase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,550,584	0	A	G	100.0%	67.5 / NA	19	S25P (TCA→CCA)	eutC	ethanolamine ammonia‑lyase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

ACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCC  >  NZ_CP009273/2550460‑2550705                                                                                                                             |                                                                                                                          aCGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCg                                                                                                             >  2:377162/1‑139 (MQ=255)        cgcgATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTcc                                                                                                      >  2:97667/1‑139 (MQ=255)               cGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGAc                                                                                               >  1:87841/1‑139 (MQ=255)                        aaCACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACg                                                                                      <  1:85680/139‑1 (MQ=255)                                            cTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAAt                                                                  <  1:377162/139‑1 (MQ=255)                                            cTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAAt                                                                  <  1:421322/139‑1 (MQ=255)                                            cTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAAt                                                                  <  2:241950/139‑1 (MQ=255)                                                                 gcgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATg                                             >  2:200163/1‑139 (MQ=255)                                                                  cgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAAt                                                                  <  1:112314/117‑1 (MQ=255)                                                                  cgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAAt                                                                  >  2:112314/1‑117 (MQ=255)                                                                  cgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGt                                            >  2:439969/1‑139 (MQ=255)                                                                                aGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGtcat                              <  1:200163/139‑1 (MQ=255)                                                                                                    ttGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCa                                                    <  1:83810/97‑1 (MQ=255)                                                                                                    ttGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCa                                                    >  2:83810/1‑97 (MQ=255)                                                                                                          gtggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATcgcccg     <  1:253397/138‑1 (MQ=255)                                                                                                          gtggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGGTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATcgcccg     <  1:439969/138‑1 (MQ=255)                                                                                                            ggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCgcccgccc  >  1:65969/1‑139 (MQ=255)                                                                                                            ggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCgcccgccc  >  1:272412/1‑139 (MQ=255)                                                                                                            ggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCgcccgccc  >  2:76995/1‑139 (MQ=255)                                                                                                                             |                                                                                                                          ACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCC  >  NZ_CP009273/2550460‑2550705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑