Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,590,774	A→C	T68T (ACT→ACG)	purC ←	phosphoribosylaminoimidazolesuccinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,590,774	0	A	C	100.0%	92.9 / NA	26	T68T (ACT→ACG)	purC	phosphoribosylaminoimidazolesuccinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/10);  total (16/10)

TCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACC  >  NZ_CP009273/2590652‑2590906                                                                                                                           |                                                                                                                                     tcttcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCtt                                                                                                                      >  1:216471/1‑139 (MQ=255)    tcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAg                                                                                                                   >  2:353444/1‑139 (MQ=255)          ccAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGt                                                                                                              <  2:62886/138‑1 (MQ=255)          ccAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGt                                                                                                              <  2:381198/138‑1 (MQ=255)                          gagagCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATg                                                                                                  >  2:297646/1‑134 (MQ=255)                          gagagCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATg                                                                                                  <  1:297646/134‑1 (MQ=255)                             agCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGtt                                                                                          >  2:316614/1‑139 (MQ=255)                               ccAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTg                                                                                         >  2:2763/1‑138 (MQ=255)                                 agcagcACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAAc                                                                                      <  1:353444/139‑1 (MQ=255)                                       aCGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                                                <  1:14245/139‑1 (MQ=255)                                              cGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCa                                                                         >  2:174132/1‑139 (MQ=255)                                                    acacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCtt                                                                   <  2:356596/139‑1 (MQ=255)                                                     cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCttt                                                                  >  1:52512/1‑139 (MQ=255)                                                     cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCttt                                                                  >  1:244360/1‑139 (MQ=255)                                                              ccGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCaaa                                                         <  2:376084/139‑1 (MQ=255)                                                               cGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAAc                                                        >  1:9211/1‑139 (MQ=255)                                                                 gCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                                                <  1:161988/113‑1 (MQ=255)                                                                 gCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                                                >  2:161988/1‑113 (MQ=255)                                                                           cAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgc                                            <  2:9211/139‑1 (MQ=255)                                                                                 tttCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                                       <  1:255660/138‑1 (MQ=255)                                                                                  ttCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCAt                                     >  1:393366/1‑139 (MQ=255)                                                                                                   aTCGGAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCa                    >  2:408499/1‑139 (MQ=255)                                                                                                      ggAGAGCAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTg                 >  2:266277/1‑139 (MQ=255)                                                                                                            cAGACGCTCCATTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAAt           >  2:244955/1‑139 (MQ=255)                                                                                                                      aTTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAATTCGAGCAcc  >  2:131548/1‑138 (MQ=255)                                                                                                                      aTTTGCGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAATTCGAGCAcc  >  1:429832/1‑138 (MQ=255)                                                                                                                           |                                                                                                                                     TCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACC  >  NZ_CP009273/2590652‑2590906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑