Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	253,379	T→C	G122G (GGT→GGC)	frsA →	esterase FrsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	253,379	0	T	C	100.0%	76.7 / NA	23	G122G (GGT→GGC)	frsA	esterase FrsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/13);  total (10/13)

TGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGC  >  NZ_CP009273/253244‑253478                                                                                                                                        |                                                                                                    tGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGgccgt                                                                                                  >  1:289518/1‑139 (MQ=255)      aaCGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTg                                                                                             >  1:341589/1‑139 (MQ=255)       aCGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTgg                                                                                            <  2:405701/139‑1 (MQ=255)          tACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTg                                                                                         <  1:34707/139‑1 (MQ=255)           aCCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTg                                                                                         >  2:248400/1‑138 (MQ=255)             cgacgaTGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                                      >  2:11884/1‑139 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                               <  1:259845/138‑1 (MQ=255)                      tttATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                                             <  1:146786/139‑1 (MQ=255)                      tttATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                                             <  2:188828/139‑1 (MQ=255)                      tttATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                                             <  1:353679/139‑1 (MQ=255)                           acgatacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTgg                                                                                            >  2:108206/1‑119 (MQ=255)                           acgatacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTgg                                                                                            <  1:108206/119‑1 (MQ=255)                               tacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGAGGCCGTCACTGGCTGCATGCGGCTACGTTGTacaa                                                                     >  2:245109/1‑138 (MQ=255)                                  ggTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACAtt                                                                 <  1:245109/139‑1 (MQ=255)                                    tGATTGGCTACCGTGGCGGCAACTGGCTTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTgc                                                               >  2:223470/1‑139 (MQ=255)                                    tGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                               >  1:277536/1‑123 (MQ=255)                                    tGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                               <  2:277536/123‑1 (MQ=255)                                    tGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTgc                                                               >  1:188891/1‑139 (MQ=255)                                              ccGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCt                                                     <  1:219974/139‑1 (MQ=255)                                              ccGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCt                                                     <  2:265446/139‑1 (MQ=255)                                                                                   gCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAgg                <  1:84627/139‑1 (MQ=255)                                                                                                aacaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg   <  2:341589/139‑1 (MQ=255)                                                                                                 acaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcgc  >  2:288069/1‑139 (MQ=255)                                                                                                 acaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcgc  >  2:307538/1‑139 (MQ=255)                                                                                                                                        |                                                                                                    TGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGC  >  NZ_CP009273/253244‑253478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑