Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,637,057	G→A	L196F (CTT→TTT)	rlmN ←	bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,637,057	0	G	A	100.0%	79.7 / NA	21	L196F (CTT→TTT)	rlmN	bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/12);  total (9/12)

GGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACAC  >  NZ_CP009273/2636943‑2637189                                                                                                                   |                                                                                                                                     gggAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAacgac                                                                                                              <  2:330269/139‑1 (MQ=255) gggAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAacgac                                                                                                              <  2:139794/139‑1 (MQ=255)                        aTCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCt                                                                                       <  2:183559/139‑1 (MQ=255)                               cGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccat                                                                                >  2:102740/1‑139 (MQ=255)                                      tttATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGc                                                                                        <  1:308694/124‑1 (MQ=255)                                      tttATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGc                                                                                        >  2:308694/1‑124 (MQ=255)                                          tCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCAtcatca                                                                     <  2:196195/139‑1 (MQ=255)                                               cgcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACg                                                                >  2:309665/1‑139 (MQ=255)                                                gcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACgttgt                                                                                                         >  2:337153/1‑97 (MQ=255)                                                gcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACgttgt                                                                                                         <  1:337153/97‑1 (MQ=255)                                                gcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGt                                                               >  2:198470/1‑139 (MQ=255)                                                         acacCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCg                                                      >  1:409648/1‑139 (MQ=255)                                                             cGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACg                                                  <  1:102740/139‑1 (MQ=255)                                                               gAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCt                                                >  2:236508/1‑139 (MQ=255)                                                                 aGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGa                                              <  1:198470/139‑1 (MQ=255)                                                                    ggAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCg                                           >  2:419659/1‑139 (MQ=255)                                                                        aGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGa                                       <  2:387176/139‑1 (MQ=255)                                                                                               gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCAtcatca                                                                     <  2:320826/86‑1 (MQ=255)                                                                                               gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCAtcatca                                                                     >  1:320826/1‑86 (MQ=255)                                                                                                    aaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgccgc           <  1:84704/139‑1 (MQ=255)                                                                                                              atcaAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCacac  <  2:350689/138‑1 (MQ=255)                                                                                                                   |                                                                                                                                     GGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACAC  >  NZ_CP009273/2636943‑2637189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑