Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,644,099	T→G	A516A (GCA→GCC)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,644,099	0	T	G	100.0%	46.4 / NA	14	A516A (GCA→GCC)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/6);  total (8/6)

AACTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGA  >  NZ_CP009273/2643965‑2644231                                                                                                                                       |                                                                                                                                     aaCTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCAc                                                                                                                                  <  1:13687/139‑1 (MQ=255)      aaCTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATa                                                                                                                              >  1:109465/1‑138 (MQ=255)                                                        cAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCg                                                                            <  1:227261/138‑1 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAg                                                   >  2:181219/1‑139 (MQ=255)                                                                                     cGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCa                                              >  2:167688/1‑139 (MQ=255)                                                                                          tGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCAc                                                                                                                                  >  1:388288/1‑50 (MQ=255)                                                                                          tGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCAc                                                                                                                                  <  2:388288/50‑1 (MQ=255)                                                                                          tGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATgcgc                                         >  1:70974/1‑139 (MQ=255)                                                                                            ggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGctc                                       <  2:109465/139‑1 (MQ=255)                                                                                                                 gCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTg                  >  1:36478/1‑139 (MQ=255)                                                                                                                         ttACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATccc          <  2:70974/139‑1 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATccc          <  1:341704/131‑1 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATccc          >  2:341704/1‑131 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGa  >  1:118907/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     AACTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGA  >  NZ_CP009273/2643965‑2644231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑