| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,666,371 | T→A | D322E (GAT→GAA) | hcaD → | phenylpropionate dioxygenase ferredoxin reductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,666,371 | 0 | T | A | 100.0% | 39.5 / NA | 11 | D322E (GAT→GAA) | hcaD | phenylpropionate dioxygenase ferredoxin reductase subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (4/7); total (4/7) | |||||||||||
TGGCAATCACTCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGT > NZ_CP009273/2666232‑2666496 | tgGCAATCACTCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGG‑‑‑‑‑‑GCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTa < 1:141316/139‑1 (MQ=255) tCACTCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTa < 1:377317/139‑1 (MQ=255) gTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGa < 1:250637/139‑1 (MQ=255) aTAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACt > 2:208131/1‑138 (MQ=255) gcgcAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAAcc > 2:182258/1‑139 (MQ=255) gggCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTa < 1:232973/139‑1 (MQ=255) accgctaccgctACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGc > 2:417429/1‑70 (MQ=255) accgctaccgctACTGCCGCCGCCGTGATTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGc < 1:417429/70‑1 (MQ=255) ccgctaccgctaCTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTg > 2:134526/1‑138 (MQ=255) gctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGc < 2:152975/139‑1 (MQ=255) gTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGt < 1:182258/138‑1 (MQ=255) | TGGCAATCACTCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAAACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGT > NZ_CP009273/2666232‑2666496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |