Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,745,770	A→G	E206G (GAG→GGG)	recN →	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,745,770	0	A	G	100.0%	53.4 / NA	16	E206G (GAG→GGG)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/7);  total (9/7)

AGTTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGAGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACA  >  NZ_CP009273/2745644‑2745895                                                                                                                               |                                                                                                                              aGTTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACaaa                                                                                                                    <  2:351686/138‑1 (MQ=255)      tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                              <  1:40029/139‑1 (MQ=255)      tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgac                                                                                                               >  2:340278/1‑138 (MQ=255)             aaaGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                              >  1:389211/1‑132 (MQ=255)             aaaGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                              <  2:389211/132‑1 (MQ=255)                                                     ggAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCAccagc                                                               <  1:340278/139‑1 (MQ=255)                                                       aaCGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCAccagcca                                                             <  1:258682/139‑1 (MQ=255)                                                       aaCGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCAccagc                                                               >  2:258682/1‑137 (MQ=255)                                                                    tGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCa                                                >  2:409373/1‑139 (MQ=255)                                                                           ctgctgCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATgg                                         >  1:14717/1‑139 (MQ=255)                                                                              ctgcAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGcc                                       >  1:101443/1‑138 (MQ=255)                                                                                           tAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACg                          >  1:69061/1‑138 (MQ=255)                                                                                              aaGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCaaa                      >  1:415451/1‑139 (MQ=255)                                                                                                      aaCGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCa                >  2:330602/1‑137 (MQ=255)                                                                                                                   cGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTaca  <  1:141148/138‑1 (MQ=255)                                                                                                                   cGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTaca  <  2:14717/138‑1 (MQ=255)                                                                                                                               |                                                                                                                              AGTTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGAGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACA  >  NZ_CP009273/2745644‑2745895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑