Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,772,143	A→G	S1315P (TCC→CCC)	ypjA ←	adhesin‑like autotransporter YpjA/EhaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,772,143	0	A	G	100.0%	57.0 / NA	17	S1315P (TCC→CCC)	ypjA	adhesin‑like autotransporter YpjA/EhaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/11);  total (6/11)

TTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACG  >  NZ_CP009273/2772022‑2772269                                                                                                                          |                                                                                                                               ttCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAgcgc                                                                                                               <  2:51375/139‑1 (MQ=255)         cACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCg                                                                                                         <  2:432010/137‑1 (MQ=255)            gACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATATCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTa                                                                                                    <  2:99069/139‑1 (MQ=255)              cacCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAAt                                                                                                  <  2:115021/139‑1 (MQ=255)                         tAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGaa                                                                                       <  2:410611/139‑1 (MQ=255)                             aaCCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat                                                                                    >  1:272538/1‑138 (MQ=255)                             aaCCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat                                                                                    >  2:264719/1‑138 (MQ=255)                                       aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTg                                                                         <  2:422729/139‑1 (MQ=255)                                          ttCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCg                                                                      <  1:264719/139‑1 (MQ=255)                                               aaCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                 <  2:373098/139‑1 (MQ=255)                                                                    aaTAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGt                                            <  1:319598/139‑1 (MQ=255)                                                                                             tccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                 <  2:194876/93‑1 (MQ=255)                                                                                             tccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                 >  1:194876/1‑93 (MQ=255)                                                                                               cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggt                 >  1:208782/1‑139 (MQ=255)                                                                                                   aTCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggtggt              >  2:300546/1‑138 (MQ=255)                                                                                                              tatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACg  >  1:188398/1‑139 (MQ=255)                                                                                                              tatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACg  <  1:300546/139‑1 (MQ=255)                                                                                                                          |                                                                                                                               TTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACG  >  NZ_CP009273/2772022‑2772269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑