Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,862,125	A→G	intergenic (‑13/+127)	nlpD ← / ← pcm	murein hydrolase activator NlpD/protein‑L‑isoaspartate O‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,862,125	0	A	G	100.0%	63.3 / NA	19	intergenic (‑13/+127)	nlpD/pcm	murein hydrolase activator NlpD/protein‑L‑isoaspartate O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

CCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCA  >  NZ_CP009273/2861991‑2862247                                                                                                                                       |                                                                                                                           ccATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGgaaaa                                                                                                                        <  2:175879/139‑1 (MQ=255)     tAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTgg                                                                                                                    >  1:353128/1‑139 (MQ=255)                aCCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAg                                                                                                         >  1:297454/1‑139 (MQ=255)                aCCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAg                                                                                                         >  2:343421/1‑139 (MQ=255)                 ccggtgccggtgGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGt                                                                                                        <  2:353128/139‑1 (MQ=255)                                     gtgtCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAgg                                                                                    <  1:109701/139‑1 (MQ=255)                                       gtCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCa                                                                                  >  1:193619/1‑139 (MQ=255)                                        tCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCa                                                                                  <  1:150930/138‑1 (MQ=255)                                                gCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATACTTACCGTGCAAGGCACCCTACTg                                                                          >  1:367200/1‑138 (MQ=255)                                                gCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAgg                                                                                    >  2:4242/1‑128 (MQ=255)                                                gCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAgg                                                                                    <  1:4242/128‑1 (MQ=255)                                                           aTAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGaa                                                                >  1:246519/1‑137 (MQ=255)                                                                 aaaCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGtt                                                        <  2:246519/139‑1 (MQ=255)                                                                       gTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGa                                                   >  2:114036/1‑138 (MQ=255)                                                                                              aCGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAAc                           <  2:297454/139‑1 (MQ=255)                                                                                                ggTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAac                                                                       >  1:90350/1‑93 (MQ=255)                                                                                                ggTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAac                                                                       <  2:90350/93‑1 (MQ=255)                                                                                                           ggCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCACGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATc                >  2:103679/1‑137 (MQ=255)                                                                                                                         aTTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCa  <  2:367200/137‑1 (MQ=255)                                                                                                                         aTTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCa  <  1:114036/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                           CCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCA  >  NZ_CP009273/2861991‑2862247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑