Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,891,751	T→G	T343P (ACC→CCC)	ygcU ←	FAD‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,891,751	0	T	G	100.0%	54.4 / NA	16	T343P (ACC→CCC)	ygcU	FAD‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/8);  total (8/8)

GAATGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGG  >  NZ_CP009273/2891618‑2891854                                                                                                                                      |                                                                                                        gAATGACCGTCCAGCACTGTGATGTCGTCGGCGTGCGGAGACTCAGTACGAATACGGTTAATAACGACTTCGTAGATTTCGTGGAGGCAGATCCAGCCGCCGGACACTTCGGTGGTAAAGCCGATGTTGCCGGgttt                                                                                                      >  2:12918/1‑137 (MQ=255) gAATGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGgttt                                                                                                      >  2:433945/1‑137 (MQ=255)      aCCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGAt                                                                                                <  2:356174/138‑1 (MQ=255)                aTGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGtt                                                                                     <  2:191518/139‑1 (MQ=255)                           gtcgGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACt                                                                          >  1:156095/1‑139 (MQ=255)                                  tGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCg                                                                   <  2:15101/139‑1 (MQ=255)                                       gAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTccc                                                              <  2:156095/139‑1 (MQ=255)                                       gAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTccc                                                              <  1:433945/139‑1 (MQ=255)                                                       cGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTa                                               >  1:191293/1‑138 (MQ=255)                                                        ggTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTaaa                                             <  2:55381/139‑1 (MQ=255)                                                                    tttCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAg                                                                               >  1:38857/1‑93 (MQ=255)                                                                    tttCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAg                                                                               <  2:38857/93‑1 (MQ=255)                                                                    tttCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGAt                                 >  2:360635/1‑139 (MQ=255)                                                                             tttCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCt                        <  2:20700/139‑1 (MQ=255)                                                                                      tGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCAcgcg               >  1:368656/1‑139 (MQ=255)                                                                                           cTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTgg           >  1:267606/1‑138 (MQ=255)                                                                                                     cGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCggg  >  1:150688/1‑137 (MQ=255)                                                                                                     cGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCggg  >  1:12586/1‑137 (MQ=255)                                                                                                                                      |                                                                                                        GAATGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGG  >  NZ_CP009273/2891618‑2891854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑