Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,931,836	A→G	D337G (GAT→GGT)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,931,836	0	A	G	100.0%	120.2 / NA	35	D337G (GAT→GGT)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

AGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCG  >  NZ_CP009273/2931701‑2931962                                                                                                                                        |                                                                                                                               aggcaggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTgg                                                                                                                             <  2:191779/139‑1 (MQ=255)    caggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCg                                                                                                                           >  1:177501/1‑138 (MQ=255)         ttGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTaaaaa                                                                                                                     >  1:161058/1‑139 (MQ=255)            tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATg                                                                                                                   >  2:356326/1‑138 (MQ=255)              taACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCa                                                                                                                 <  2:282633/138‑1 (MQ=255)                 cccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCa                                                                                                                 >  1:364046/1‑135 (MQ=255)                 cccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCa                                                                                                                 <  2:364046/135‑1 (MQ=255)                   cAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGa                                                                                                           >  2:207279/1‑139 (MQ=255)                      gTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGAtt                                                                                                         >  1:187389/1‑138 (MQ=255)                      gTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGAtt                                                                                                         >  1:23116/1‑138 (MQ=255)                      gTATGCAATGGCTGGCATCCGGAGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGAtt                                                                                                         >  1:102738/1‑138 (MQ=255)                           cAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGt                                                                                                   >  1:2469/1‑139 (MQ=255)                                       tCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACg                                                                                       <  2:161058/139‑1 (MQ=255)                                                        ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                      <  1:356326/139‑1 (MQ=255)                                                        ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                      <  1:389858/139‑1 (MQ=255)                                                             gaAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCt                                                                 >  1:230772/1‑139 (MQ=255)                                                                    gTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCa                                                                                                                 >  2:388087/1‑84 (MQ=255)                                                                    gTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTCATCGCGCCTGGCGCGGGTGGCGTAAAAATGCa                                                                                                                 <  1:388087/84‑1 (MQ=255)                                                                           aCGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggg                                                   <  1:358898/139‑1 (MQ=255)                                                                             ggCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTgg                                                                                                                             <  2:73024/63‑1 (MQ=255)                                                                             ggCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTgg                                                                                                                             >  1:73024/1‑63 (MQ=255)                                                                                        ccTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCAt                                              <  1:134922/131‑1 (MQ=255)                                                                                        ccTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCAt                                              >  2:134922/1‑131 (MQ=255)                                                                                         cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgc                                     >  1:246437/1‑139 (MQ=255)                                                                                            gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgcgcg                                  >  2:76192/1‑139 (MQ=255)                                                                                                 tGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggg                                                   <  1:406710/117‑1 (MQ=255)                                                                                                 tGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggg                                                   >  2:406710/1‑117 (MQ=255)                                                                                                 tGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCt                             >  2:362650/1‑139 (MQ=255)                                                                                                     gattgaAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGa                          <  1:234884/138‑1 (MQ=255)                                                                                                             aagCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAAc                 <  2:246437/139‑1 (MQ=255)                                                                                                             aagCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAAc                 <  1:32575/139‑1 (MQ=255)                                                                                                                   gTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTgcg             <  2:102738/137‑1 (MQ=255)                                                                                                                         tCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACa     <  2:2469/139‑1 (MQ=255)                                                                                                                         tCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACa     <  1:207279/139‑1 (MQ=255)                                                                                                                            cgcCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg  <  1:362650/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                               AGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCG  >  NZ_CP009273/2931701‑2931962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑