Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,959,152	A→G	F82S (TTC→TCC)	lgt ←	prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,959,152	0	A	G	100.0%	27.2 / NA	9	F82S (TTC→TCC)	lgt	prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

CTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATT  >  NZ_CP009273/2959019‑2959286                                                                                                                                      |                                                                                                                                       cTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTg                                                                                                                                   <  2:339808/139‑1 (MQ=255)      agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaa                                                                                                                              <  1:361701/139‑1 (MQ=255)      agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaa                                                                                                                              <  2:3/139‑1 (MQ=255)                                            accacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGcc                                                                                          >  2:181382/1‑137 (MQ=255)                                                                                 tGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAttttt                                                   >  1:430832/1‑139 (MQ=255)                                                                                                     aaCAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGAc                               >  1:129551/1‑139 (MQ=255)                                                                                                     aaCAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGAc                               >  1:168627/1‑139 (MQ=255)                                                                                                       cAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGa                             <  2:175616/139‑1 (MQ=255)                                                                                                                                  cGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACAtt  >  2:336096/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       CTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATT  >  NZ_CP009273/2959019‑2959286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑