Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,071,778	A→C	G147G (GGT→GGG)	cmtA ←	PTS mannitol transporter subunit IICB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,071,778	0	A	C	100.0%	109.1 / NA	31	G147G (GGT→GGG)	cmtA	PTS mannitol transporter subunit IICB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/19);  total (12/19)

CATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCAT  >  NZ_CP009273/3071642‑3071909                                                                                                                                         |                                                                                                                                    cATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAccc                                                                                                                                   >  1:373042/1‑139 (MQ=255)     attGAGGAAAAGCACTTTCGCCGGTTCATGGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcag                                                                                                                                <  2:229365/138‑1 (MQ=255)                     ttCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATcc                                                                                                               >  2:36186/1‑139 (MQ=255)                         ccGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCGGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                                                                           <  2:2565/139‑1 (MQ=255)                          cGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgcg                                                                                                          <  1:219748/139‑1 (MQ=255)                                       actgacAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCTGACAAAGGAGCATCCCCGCGATACCTAATgag                                                                                              <  1:391418/138‑1 (MQ=255)                                           acaacaATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAg                                                                                           <  1:36186/137‑1 (MQ=255)                                           acaacaATGGCAGATAACCCCCATGTCCCAGCGCCTAAATACACTCTTTGCCGAAAGTATTGGCAATTAACCCCGCCGGGCCGATAACTTCAAACCCCAGCATACAAAGGAGCATCTCCGCGATACCTAATGAGAAGt                                                                                          <  2:330937/138‑1 (MQ=255)                                                                cATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAAcc                                                                    >  1:148948/1‑139 (MQ=255)                                                                cATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAAcc                                                                    >  1:197359/1‑139 (MQ=255)                                                                    tACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCgg                                                                  >  2:21091/1‑137 (MQ=255)                                                                         gcgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTAt                                                           >  2:273509/1‑139 (MQ=255)                                                                         gcgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTAt                                                           >  2:157977/1‑139 (MQ=255)                                                                          cgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATg                                                          >  1:22014/1‑139 (MQ=255)                                                                              tCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCAt                                                                                                                 >  2:85605/1‑80 (MQ=255)                                                                              tCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCAt                                                                                                                 <  1:85605/80‑1 (MQ=255)                                                                                    cacTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCa                                                <  2:115548/139‑1 (MQ=255)                                                                                         tttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCaaa                                                                       >  2:5821/1‑111 (MQ=255)                                                                                         tttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCaaa                                                                       <  1:5821/111‑1 (MQ=255)                                                                                                 aaGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGa                                    >  2:118730/1‑138 (MQ=255)                                                                                                  aGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACa                                  <  1:157977/139‑1 (MQ=255)                                                                                                          cAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTAt                          <  1:118730/139‑1 (MQ=255)                                                                                                          cAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTAt                          <  1:273509/139‑1 (MQ=255)                                                                                                              tAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGAc                       >  1:305438/1‑138 (MQ=255)                                                                                                                   ccgccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAAcc                  >  2:16555/1‑138 (MQ=255)                                                                                                                        gggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCG‑TTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg           <  2:101546/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg           <  2:180041/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg           <  1:21091/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg           <  2:373042/139‑1 (MQ=255)                                                                                                                         ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg           <  2:41723/139‑1 (MQ=255)                                                                                                                             gATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGc       <  2:262406/139‑1 (MQ=255)                                                                                                                                  cTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCAt  <  2:159217/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCAT  >  NZ_CP009273/3071642‑3071909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑