Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,148,355	A→G	S56P (TCC→CCC)	yqhC ←	DNA‑binding transcriptional regulator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,148,355	0	A	G	100.0%	55.9 / NA	17	S56P (TCC→CCC)	yqhC	DNA‑binding transcriptional regulator YqhC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/7);  total (10/7)

CTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATT  >  NZ_CP009273/3148229‑3148466                                                                                                                               |                                                                                                                cTGCCAGCGGCACCGCTGACGGGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTat                                                                                                     <  2:269262/139‑1 (MQ=255)    ccAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTatgat                                                                                                  >  2:430150/1‑139 (MQ=255)      aGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCATCGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGc                                                                                                <  2:137800/139‑1 (MQ=255)                        gCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCg                                                                              >  2:86251/1‑139 (MQ=255)                                  cGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATc                                                                                  >  1:68090/1‑125 (MQ=255)                                  cGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATc                                                                                  <  2:68090/125‑1 (MQ=255)                                             ggcaacggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGtct                                                         <  1:430150/139‑1 (MQ=255)                                                 acggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGc                                                      >  2:383365/1‑138 (MQ=255)                                                     caCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATa                                                  >  2:114173/1‑138 (MQ=255)                                                                   ggTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATc                                   <  2:376062/139‑1 (MQ=255)                                                                    gTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCg                                  >  2:115454/1‑139 (MQ=255)                                                                        tCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCAc                                                                                >  2:340119/1‑89 (MQ=255)                                                                        tCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCAc                                                                                <  1:340119/89‑1 (MQ=255)                                                                          aTTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCTCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                             <  1:412840/138‑1 (MQ=255)                                                                                             acGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAtttt         >  2:336269/1‑139 (MQ=255)                                                                                             acGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAtttt         >  2:65381/1‑139 (MQ=255)                                                                                                    cATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTAtt  >  1:367883/1‑139 (MQ=255)                                                                                                                               |                                                                                                                CTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATT  >  NZ_CP009273/3148229‑3148466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑