Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,155,922	A→G	V36A (GTT→GCT)	ftsP ←	cell division protein FtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,155,922	0	A	G	100.0%	73.9 / NA	22	V36A (GTT→GCT)	ftsP	cell division protein FtsP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

ATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGC  >  NZ_CP009273/3155816‑3156050                                                                                                           |                                                                                                                                 aTTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                                                                  <  2:53755/139‑1 (MQ=255) aTTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                                                                  >  1:67726/1‑139 (MQ=255) aTTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                                                                  >  1:298338/1‑139 (MQ=255)  ttGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTgg                                                                                                 >  1:439650/1‑139 (MQ=255)                   cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAgcg                                                                                >  2:299437/1‑139 (MQ=255)                                  cGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCa                                                                 <  2:160640/139‑1 (MQ=255)                                      aaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCtt                                                                                             <  2:177635/107‑1 (MQ=255)                                      aaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCtt                                                                                             >  1:177635/1‑107 (MQ=255)                                      aaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATcc                                                             >  1:216460/1‑139 (MQ=255)                                               tGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCt                                                    >  1:257154/1‑139 (MQ=255)                                                              gTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGcc                                     <  2:298338/139‑1 (MQ=255)                                                               tCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGcc                                     >  2:259749/1‑138 (MQ=255)                                                                  tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt                                 <  1:236089/139‑1 (MQ=255)                                                                  tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt                                 <  2:439650/139‑1 (MQ=255)                                                                    aaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGAGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt                                 >  1:80993/1‑137 (MQ=255)                                                                                                 tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  <  1:289721/139‑1 (MQ=255)                                                                                                 tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  <  1:286911/139‑1 (MQ=255)                                                                                                 tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  <  1:258917/139‑1 (MQ=255)                                                                                                 tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  <  1:2329/139‑1 (MQ=255)                                                                                                 tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  <  2:67726/139‑1 (MQ=255)                                                                                                    cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  <  2:75950/136‑1 (MQ=255)                                                                                                    cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc  >  1:75950/1‑136 (MQ=255)                                                                                                           |                                                                                                                                 ATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGC  >  NZ_CP009273/3155816‑3156050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑