Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,193,900	A→G	L142P (CTC→CCC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,193,900	0	A	G	100.0%	76.0 / NA	23	L142P (CTC→CCC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/6);  total (17/6)

CGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTAA  >  NZ_CP009273/3193771‑3194033                                                                                                                                  |                                                                                                                                      cGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGAttt                                                                                                                              >  2:379226/1‑139 (MQ=255)     aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAt                                                                                                                          >  1:392906/1‑139 (MQ=255)                            ttCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGccac                                                                                                     >  2:285416/1‑137 (MQ=255)                                             cgcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATa                                                                                   >  1:57415/1‑138 (MQ=255)                                             cgcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGACGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATa                                                                                   >  1:374251/1‑138 (MQ=255)                                                 cgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTTACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  2:290443/139‑1 (MQ=255)                                                       tCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc                                                                        >  2:156295/1‑139 (MQ=255)                                                                 gCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCgg                                                              <  1:156295/139‑1 (MQ=255)                                                                     ttccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  1:426536/119‑1 (MQ=255)                                                                     ttccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              >  2:426536/1‑119 (MQ=255)                                                                     ttccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTg                                                           >  1:368984/1‑138 (MQ=255)                                                                     ttccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTg                                                           >  2:302016/1‑138 (MQ=255)                                                                       ccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              >  1:118038/1‑117 (MQ=255)                                                                       ccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  2:118038/117‑1 (MQ=255)                                                                       ccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAc                                                        >  2:435069/1‑139 (MQ=255)                                                                               agCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAgg                                                >  1:215866/1‑139 (MQ=255)                                                                               agCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGAACGCGGGAgg                                                >  1:190340/1‑139 (MQ=255)                                                                                                       tCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGt                        >  1:260150/1‑139 (MQ=255)                                                                                                                 tCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGAc              >  1:21234/1‑139 (MQ=255)                                                                                                                   aCTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACtt            <  1:302016/139‑1 (MQ=255)                                                                                                                        ccccTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTc       >  1:237629/1‑139 (MQ=255)                                                                                                                         cccTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCg      >  1:95630/1‑139 (MQ=255)                                                                                                                              gTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTaa  <  2:260150/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      CGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTAA  >  NZ_CP009273/3193771‑3194033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑