| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 453,695 | G→A | A272T (GCG→ACG) | clpX → | ATP‑dependent protease ATP‑binding subunit ClpX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 453,695 | 0 | G | A | 93.3% | 41.9 / ‑3.7 | 16 | A272T (GCG→ACG) | clpX | ATP‑dependent protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (9/5); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
CCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAAC > NZ_CP009273/453572‑453830 | ccGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTGCGGGCTGGGTAAAAGGATTTTCCACCGTGTAGAAAACGGCTCCGGCATTGGGTTTGGCACGCAGGTAAAAACg > 2:398248/1‑138 (MQ=255) cGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGaa < 1:12164/139‑1 (MQ=255) aTACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGa < 2:283481/139‑1 (MQ=255) cTGTTTATTTGTGGCGGTGCGGTTTCCGGACTGGGTTAAGGGGTTTTCCCCCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGGAAAAGCGAAATCCGACAAAGCAAGGGGAGGCGAGGTGCCGGGGCAgg > 1:299947/1‑139 (MQ=255) tGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGAGAAAGTGATTTTCCACCGTGTTGAAACCGGCTCCGGCATTGGGTTTTGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt > 1:424488/1‑139 (MQ=255) tATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTTCCACCGTGTAGAAAACGGCCCCGGGATTGGGTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGaa > 1:159703/1‑139 (MQ=255) ccGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGC‑CGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 1:399950/138‑1 (MQ=255) tCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGt > 1:291274/1‑139 (MQ=255) ccGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGgt < 1:339364/139‑1 (MQ=255) gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGgtcg > 2:314926/1‑139 (MQ=255) aCCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCgg > 1:382140/1‑139 (MQ=255) aCCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCgg > 1:60355/1‑139 (MQ=255) gCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGt > 2:346362/1‑139 (MQ=255) cGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCg < 2:291274/137‑1 (MQ=255) gCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc < 1:309400/139‑1 (MQ=255) ggCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAAc > 2:417303/1‑139 (MQ=255) | CCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAAC > NZ_CP009273/453572‑453830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |