Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,315,373	T→C	intergenic (‑401/+58)	yhbX ← / ← BW25113_RS16455	phosphoethanolamine transferase/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,315,373	0	T	C	100.0%	100.1 / NA	29	intergenic (‑401/+58)	yhbX/BW25113_RS16455	phosphoethanolamine transferase/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

GACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCAC  >  NZ_CP009273/3315245‑3315508                                                                                                                                 |                                                                                                                                        gACACAATAAAGTGCCAATTATGTCAGTAGAAGGGGAAAATTTATAACTAAGGCGTACCGGCACAATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGAc                                                                                                                                 >  1:187850/1‑137 (MQ=255) gACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGAcc                                                                                                                                <  1:408536/138‑1 (MQ=255) gACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGAc                                                                                                                                 >  1:382409/1‑137 (MQ=255) gACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGAc                                                                                                                                 >  1:207884/1‑137 (MQ=255)      aaTAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGAc                                                                                                                           >  1:126516/1‑138 (MQ=255)                                             taACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                   <  2:382409/139‑1 (MQ=255)                                                            ggCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                    <  2:436577/139‑1 (MQ=255)                                                            ggCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                    <  1:359624/139‑1 (MQ=255)                                                                  aTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                              >  1:197797/1‑139 (MQ=255)                                                                  aTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                              <  2:406585/139‑1 (MQ=255)                                                                            ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGt                                                    <  1:244763/139‑1 (MQ=255)                                                                                    cTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTa                                            <  2:207884/139‑1 (MQ=255)                                                                                               gCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 <  2:126516/139‑1 (MQ=255)                                                                                               gCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 <  2:374396/139‑1 (MQ=255)                                                                                               gCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 <  2:356687/139‑1 (MQ=255)                                                                                                   cTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                             <  2:314416/139‑1 (MQ=255)                                                                                                      gACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTc                           <  2:386916/138‑1 (MQ=255)                                                                                                                     ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 <  2:72993/117‑1 (MQ=255)                                                                                                                     ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 >  1:72993/1‑117 (MQ=255)                                                                                                                     ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc           >  2:90182/1‑139 (MQ=255)                                                                                                                     ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc           >  2:157548/1‑139 (MQ=255)                                                                                                                     ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc           >  1:261832/1‑139 (MQ=255)                                                                                                                     ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc           >  1:221830/1‑139 (MQ=255)                                                                                                                      cTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc          <  2:123308/139‑1 (MQ=255)                                                                                                                       tGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa            >  2:256743/1‑136 (MQ=255)                                                                                                                       tGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa            <  1:256743/136‑1 (MQ=255)                                                                                                                        gACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 <  1:88637/114‑1 (MQ=255)                                                                                                                        gACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                 >  2:88637/1‑114 (MQ=255)                                                                                                                              cATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac  <  1:256843/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        GACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCAC  >  NZ_CP009273/3315245‑3315508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑