| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,326,882 | A→G | intergenic (‑72/‑187) | rplU ← / → ispB | 50S ribosomal protein L21/octaprenyl diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,326,882 | 0 | A | G | 97.6% | 141.7 / ‑5.6 | 42 | intergenic (‑72/‑187) | rplU/ispB | 50S ribosomal protein L21/octaprenyl diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (21/20); minor base C (0/1); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAT > NZ_CP009273/3326766‑3327008 | gCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCt > 1:68224/1‑139 (MQ=255)gCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCt > 1:432099/1‑139 (MQ=255) cTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc < 1:132352/139‑1 (MQ=255) cTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc < 1:422798/139‑1 (MQ=255) ttgttTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCt < 2:221946/139‑1 (MQ=255) tttACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTAc < 2:67719/138‑1 (MQ=255) cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATacac > 2:323393/1‑139 (MQ=255) tGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACNNg > 2:133638/1‑137 (MQ=255) cgTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc > 1:125517/1‑106 (MQ=255) cgTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc < 2:125517/106‑1 (MQ=255) cgTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaaaa > 1:254691/1‑138 (MQ=255) tACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAacac < 1:285815/139‑1 (MQ=255) ccGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGt > 1:404566/1‑138 (MQ=255) cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGt < 1:33547/132‑1 (MQ=255) cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGCCAATACACGAAGAAAAAAAACACAACTTGt > 2:33547/1‑132 (MQ=255) gCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAAc > 1:206757/1‑139 (MQ=255) cgcgCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTa > 2:24232/1‑139 (MQ=255) acCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGc > 2:267271/1‑139 (MQ=255) ttCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAt < 2:432099/137‑1 (MQ=255) ttCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAt < 2:254691/137‑1 (MQ=255) ttCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAt < 2:68224/137‑1 (MQ=255) aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAAc > 2:165067/1‑103 (MQ=255) aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAAc < 1:165067/103‑1 (MQ=255) agTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc < 1:399646/139‑1 (MQ=255) agTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc < 2:404566/139‑1 (MQ=255) agTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc < 1:24232/139‑1 (MQ=255) gTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa < 1:323393/139‑1 (MQ=255) tGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAc > 2:257071/1‑139 (MQ=255) cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAccc > 1:88148/1‑139 (MQ=255) cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAccc > 2:301031/1‑139 (MQ=255) tataAATATTCACAATAGGCCGCGCATATTCCACAAAACGCACCCCTTTGACAAGTCCTACAGTCATTACTCAAAAAAAAAAAACACAACTTGTACGTTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCt < 1:133638/137‑1 (MQ=255) taAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCtat < 2:206757/137‑1 (MQ=255) atacacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCt < 1:285434/127‑1 (MQ=255) atTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAACAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCt > 2:285434/1‑130 (MQ=255) atTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCt > 1:17950/1‑138 (MQ=255) cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTa > 1:311746/1‑85 (MQ=255) cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTa < 2:311746/85‑1 (MQ=255) cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAc > 1:355613/1‑138 (MQ=255) cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAc > 1:375694/1‑138 (MQ=255) caATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt < 2:336518/139‑1 (MQ=255) cgcgGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc > 1:194512/1‑112 (MQ=255) cgcgGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc < 2:194512/112‑1 (MQ=255) | GCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAT > NZ_CP009273/3326766‑3327008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |