Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,349,491	T→C	S470P (TCC→CCC)	gltB →	glutamate synthase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,349,491	0	T	C	100.0%	116.9 / NA	33	S470P (TCC→CCC)	gltB	glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/14);  total (19/14)

GTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACT  >  NZ_CP009273/3349355‑3349625                                                                                                                                         |                                                                                                                                       gTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGAcccc                                                                                                                                      >  2:229023/1‑139 (MQ=255)   ggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCg                                                                                                                                     >  2:1574/1‑138 (MQ=255)    gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGTGCGGAAGAGCTGGACCCCGt                                                                                                                                    <  1:109771/138‑1 (MQ=255)    gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    <  1:229023/138‑1 (MQ=255)    gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    >  1:23327/1‑138 (MQ=255)    gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    >  2:202572/1‑138 (MQ=255)    gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    <  1:334340/138‑1 (MQ=255)    gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    <  1:351429/138‑1 (MQ=255)          gaAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    >  2:88478/1‑132 (MQ=255)          gaAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGt                                                                                                                                    <  1:88478/132‑1 (MQ=255)          gaAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTcgc                                                                                                                             >  2:374910/1‑139 (MQ=255)                                                   aTGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATggg                                                                                    <  2:255721/139‑1 (MQ=255)                                                    tGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGc                                                                                   >  1:179664/1‑139 (MQ=255)                                                        gaagTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCgatg                                                                               <  2:10693/139‑1 (MQ=255)                                                              ggTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGcggtcg                                                                                              <  2:215134/118‑1 (MQ=255)                                                              ggTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGcggtcg                                                                                              >  1:215134/1‑118 (MQ=255)                                                              ggTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATAccc                                                                         >  1:301720/1‑139 (MQ=255)                                                                      cgAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGcc                                                                 >  2:188067/1‑139 (MQ=255)                                                                      cgAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGcc                                                                 >  2:118178/1‑139 (MQ=255)                                                                             gacgacgacACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGctct                                                          >  2:55672/1‑139 (MQ=255)                                                                             gacgacgacACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGct                                                            <  1:151510/137‑1 (MQ=255)                                                                                  cgacACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAga                                                     >  1:391691/1‑138 (MQ=255)                                                                                  cgacACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGt                                                     >  2:1819/1‑139 (MQ=255)                                                                                             ccAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCat                                          <  1:55672/139‑1 (MQ=255)                                                                                             ccAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                                           <  1:374910/138‑1 (MQ=255)                                                                                                           cAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATggg                                                                                    <  1:828/83‑1 (MQ=255)                                                                                                           cAGTTTAACTACAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATggg                                                                                    >  2:828/1‑83 (MQ=255)                                                                                                                      cAGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCcagcag                 >  1:328981/1‑139 (MQ=255)                                                                                                                       aGCGCGGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGt                <  2:301720/139‑1 (MQ=255)                                                                                                                           cgGAAGAGCTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGc            >  2:307868/1‑139 (MQ=255)                                                                                                                                   cTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                                           <  1:339362/100‑1 (MQ=255)                                                                                                                                   cTGGACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                                           >  2:339362/1‑100 (MQ=255)                                                                                                                                      gACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACt  >  2:172017/1‑138 (MQ=255)                                                                                                                                      gACCCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACt  >  2:46403/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                       GTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACT  >  NZ_CP009273/3349355‑3349625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑