Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,435,146	A→C	G104G (GGT→GGG)	rpsK ←	30S ribosomal protein S11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,435,146	0	A	C	100.0%	94.5 / NA	28	G104G (GGT→GGG)	rpsK	30S ribosomal protein S11
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/13);  total (15/13)

TAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAAC  >  NZ_CP009273/3435013‑3435278                                                                                                                                      |                                                                                                                                     tAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTgc                                                                                                                                 >  2:286299/1‑139 (MQ=255)   ggACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTgcgg                                                                                                                               >  2:383556/1‑139 (MQ=255)       ccAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTgcggcgat                                                                                                                           <  2:257270/139‑3 (MQ=255)         aaaTATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGtt                                                                                                                           >  2:439439/1‑137 (MQ=255)         aaaTATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGtt                                                                                                                           >  1:268322/1‑137 (MQ=255)                  gCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCa                                                                                                                    <  1:371267/135‑1 (MQ=255)                        ttctttctCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAAtag                                                                                                             >  1:336312/1‑136 (MQ=255)                                  aacaaACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGcc                                                                                                <  1:267260/139‑1 (MQ=255)                                            gAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCGCATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGa                                                                                      >  2:238759/1‑139 (MQ=255)                                            gAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCg                                                                                        <  1:439439/137‑1 (MQ=255)                                                    ggCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAAc                                                                              >  2:350225/1‑139 (MQ=255)                                                                gACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTc                                                                     >  1:90540/1‑136 (MQ=255)                                                                      ttttCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCtt                                                            >  1:182963/1‑139 (MQ=255)                                                                        ttCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTg                                                           <  2:90540/138‑1 (MQ=255)                                                                             cggACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCg                                                     <  2:105457/139‑1 (MQ=255)                                                                                               aGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGt                                     >  1:274581/1‑137 (MQ=255)                                                                                                  gATCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAgcg                                <  1:173130/139‑1 (MQ=255)                                                                                                    tCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCAt                                                                           <  2:135494/94‑1 (MQ=255)                                                                                                    tCGGAGTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCAt                                                                           >  1:135494/1‑94 (MQ=255)                                                                                                         gTCACATCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCATATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGct                         <  1:136833/139‑1 (MQ=255)                                                                                                              aTCAGTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTctgct                    <  2:182963/139‑1 (MQ=255)                                                                                                                  gTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCaa                >  1:438858/1‑139 (MQ=255)                                                                                                                  gTAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCaa                >  1:220858/1‑139 (MQ=255)                                                                                                                   tAATGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAAc               <  2:220858/139‑1 (MQ=255)                                                                                                                     aTGTTAGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCt             <  1:232362/139‑1 (MQ=255)                                                                                                                          aGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGct                         >  2:118909/1‑122 (MQ=255)                                                                                                                          aGTGATGCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGct                         <  1:118909/122‑1 (MQ=255)                                                                                                                                gCGGAACCCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAAc  >  2:283966/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                     TAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAAC  >  NZ_CP009273/3435013‑3435278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑