Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,437,182	A→G	I92T (ATC→ACC)	secY ←	preprotein translocase subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,437,182	0	A	G	100.0%	130.3 / NA	36	I92T (ATC→ACC)	secY	preprotein translocase subunit SecY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

ATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTG  >  NZ_CP009273/3437053‑3437307                                                                                                                                  |                                                                                                                              aTACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATc                                                                                                                       >  2:412097/1‑138 (MQ=255)  tACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGa                                                                                                                     <  1:306037/139‑1 (MQ=255)     cGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGc                                                                                                                  >  1:108387/1‑139 (MQ=255)      gATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                 >  1:2164/1‑139 (MQ=255)                         aGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATcc                                                                                              >  2:344895/1‑139 (MQ=255)                          gCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATccc                                                                                             >  2:44310/1‑139 (MQ=255)                                gagTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGc                                                                                       <  2:108387/139‑1 (MQ=255)                                     ccGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCa                                                                                      >  1:98645/1‑135 (MQ=255)                                        tAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGa                                                                                  >  2:432972/1‑136 (MQ=255)                                        tAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGa                                                                                  >  1:235796/1‑136 (MQ=255)                                        tAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAg                                                                               <  1:412097/139‑1 (MQ=255)                                             ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                          >  2:279221/1‑139 (MQ=255)                                             ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                          <  1:432972/139‑1 (MQ=255)                                             ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                          >  1:250513/1‑139 (MQ=255)                                                gtACTGGCTGATCTTACGACGACCAGACTCCCTTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACgg                                                                       >  1:385279/1‑139 (MQ=255)                                                   cTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCt                                                                     <  1:254435/138‑1 (MQ=255)                                                       cTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGagag                                                                <  1:248102/139‑1 (MQ=255)                                                           tCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                          >  1:255846/1‑125 (MQ=255)                                                           tCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                          <  2:255846/125‑1 (MQ=255)                                                               acgacgACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcacc                                                            <  1:44310/135‑1 (MQ=255)                                                                   cgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                    <  1:279221/139‑1 (MQ=255)                                                                   cgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                    <  2:385279/139‑1 (MQ=255)                                                                    gacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                    >  2:397140/1‑138 (MQ=255)                                                                     acCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                    <  1:33092/137‑1 (MQ=255)                                                                     acCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                    >  2:33092/1‑137 (MQ=255)                                                                     acCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGaa                                                   >  1:129612/1‑138 (MQ=255)                                                                              cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                          <  2:129612/138‑1 (MQ=255)                                                                              cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                          >  1:35829/1‑138 (MQ=255)                                                                                      tcttAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACAtctc                                    <  1:11307/136‑1 (MQ=255)                                                                                          aaTTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAatgat                              <  1:139032/138‑1 (MQ=255)                                                                                          aaTTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAatga                               <  1:373348/137‑1 (MQ=255)                                                                                                tGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcac                                                             >  1:436679/1‑101 (MQ=255)                                                                                                tGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcac                                                             <  2:436679/101‑1 (MQ=255)                                                                                                  ccAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                       <  2:235796/137‑1 (MQ=255)                                                                                                      cGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGt                 >  2:157128/1‑139 (MQ=255)                                                                                                                     cGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTg  >  2:224756/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                              ATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTG  >  NZ_CP009273/3437053‑3437307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑