Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,479,155	A→G	V8A (GTC→GCC)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,479,155	0	A	G	100.0%	75.6 / NA	23	V8A (GTC→GCC)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

ACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACA  >  NZ_CP009273/3479023‑3479284                                                                                                                                     |                                                                                                                                  acaTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACt                                                                                                                             >  2:63541/1‑139 (MQ=255)   aTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACtt                                                                                                                            >  2:422042/1‑138 (MQ=255)   aTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACtt                                                                                                                            >  2:348099/1‑138 (MQ=255)   aTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACtt                                                                                                                            >  2:204210/1‑138 (MQ=255)                    caccCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          <  1:119633/139‑1 (MQ=255)                    caccCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          <  1:233294/139‑1 (MQ=255)                    caccCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          <  2:391154/139‑1 (MQ=255)                      cccGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTGTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAACAGTTAACCCTTCGGCCCACGTCACTCGCGCTTGCATTTTTg                                                                                                        >  2:159590/1‑139 (MQ=255)                          ccgccATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTAc                                                                                                    >  2:214366/1‑139 (MQ=255)                              cATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCa                                                                                                <  1:250961/139‑1 (MQ=255)                                  aaCACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGc                                                                                            <  1:219877/139‑1 (MQ=255)                                        aTTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAAt                                                                                      >  2:166909/1‑139 (MQ=255)                                                 ggACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGa                                                                              >  1:71299/1‑138 (MQ=255)                                                              tATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGt                                                                  <  1:204210/137‑1 (MQ=255)                                                              tATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGt                                                                  <  1:422042/137‑1 (MQ=255)                                                              tATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTa                                                                 <  2:41653/138‑1 (MQ=255)                                                                   ccTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTAc                                                                      <  2:259626/128‑1 (MQ=255)                                                                   ccTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTAc                                                                      >  1:259626/1‑128 (MQ=255)                                                                       aGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCCTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTTACCAAAt                                                       >  2:132359/1‑139 (MQ=255)                                                                                 cATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACg                                              <  1:63541/138‑1 (MQ=255)                                                                                                          gATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGc                    >  1:306061/1‑139 (MQ=255)                                                                                                                 ccAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAAGCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGacac             <  1:401187/139‑1 (MQ=255)                                                                                                                             aCCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGAcaca  <  2:48428/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  ACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACA  >  NZ_CP009273/3479023‑3479284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑