Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,516,705	T→C	I159T (ATC→ACC)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,516,705	0	T	C	100.0%	90.5 / NA	26	I159T (ATC→ACC)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

CGTCACAAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCT  >  NZ_CP009273/3516570‑3516834                                                                                                                                        |                                                                                                                                  cggcacaAGGCGCAATTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGGCGAGACCCt                                                                                                                                <  2:3604/136‑1 (MQ=255) cgTCACAAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCt                                                                                                                                <  2:223894/139‑1 (MQ=255) cgTCACAAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCt                                                                                                                                <  2:397423/139‑1 (MQ=255)      caAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGc                                                                                                                           <  1:25705/139‑1 (MQ=255)            gCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATc                                                                                                                     >  2:409432/1‑139 (MQ=255)                             gcagcTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCtt                                                                                                    >  2:285297/1‑139 (MQ=255)                              cagcTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTg                                                                                                   >  1:140030/1‑139 (MQ=255)                                   ggCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTAc                                                                                              >  1:83967/1‑139 (MQ=255)                                    gCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTAc                                                                                              <  1:380146/138‑1 (MQ=255)                                    gCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTAc                                                                                              <  1:44153/138‑1 (MQ=255)                                     cGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACcg                                                                                            <  1:106508/139‑1 (MQ=255)                                           aCTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTa                                                                                      <  2:55233/139‑1 (MQ=255)                                                  ccTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGgtgt                                                                                <  2:35569/138‑1 (MQ=255)                                                          cAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGtgctg                                                                        <  2:348912/138‑1 (MQ=255)                                                                  acgcTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAg                                                               >  1:37646/1‑139 (MQ=255)                                                                  acgcTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAg                                                               >  1:331229/1‑139 (MQ=255)                                                                       gATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTAt                                                          >  2:94704/1‑139 (MQ=255)                                                                                          gTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTAt                                                                                     >  2:96584/1‑93 (MQ=255)                                                                                          gTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTAt                                                                                     <  1:96584/93‑1 (MQ=255)                                                                                           tCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGAcc                                       >  1:427797/1‑138 (MQ=255)                                                                                           tCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGAcc                                       >  1:336539/1‑138 (MQ=255)                                                                                                 tCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGAc                                >  2:291064/1‑139 (MQ=255)                                                                                                           cATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGaa                      <  1:208434/139‑1 (MQ=255)                                                                                                                          gACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGcc       >  2:276260/1‑139 (MQ=255)                                                                                                                               aaGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCt  >  2:115541/1‑139 (MQ=255)                                                                                                                               aaGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCt  >  1:298069/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                  CGTCACAAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCT  >  NZ_CP009273/3516570‑3516834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑