Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,589,221	A→G	G181G (GGT→GGC)	livH ←	high‑affinity branched‑chain amino acid ABC transporter permease LivH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,589,221	0	A	G	100.0%	128.0 / NA	37	G181G (GGT→GGC)	livH	high‑affinity branched‑chain amino acid ABC transporter permease LivH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

AGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAA  >  NZ_CP009273/3589085‑3589354                                                                                                                                         |                                                                                                                                      aGAACTGACCGAGCACCACCCCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGcc                                                                                                                                     <  2:93214/139‑1 (MQ=255)   aaCTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCa                                                                                                                                   <  1:161481/139‑1 (MQ=255)        aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGcc                                                                                                                                     <  1:32061/132‑1 (MQ=255)        aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  2:17720/1‑139 (MQ=255)        aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  1:38292/1‑139 (MQ=255)        aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGCCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGcc                                                                                                                                     >  2:32061/1‑132 (MQ=255)             gcagcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGaa                                                                                                                           >  2:156952/1‑137 (MQ=255)             gcagcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATa                                                                                                                         <  1:7218/139‑1 (MQ=255)                 cacaCCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCg                                                                                                                      >  2:208488/1‑138 (MQ=255)                                            gcCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCg                                                                                          <  1:434022/139‑1 (MQ=255)                                                    caAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGt                                                                                  <  1:312039/139‑1 (MQ=255)                                                       aGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                >  2:294718/1‑138 (MQ=255)                                                            aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATcc                                                                          <  1:294718/139‑1 (MQ=255)                                                            aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATc                                                                           >  1:342973/1‑138 (MQ=255)                                                                   tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   >  1:352849/1‑139 (MQ=255)                                                                   tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   >  2:247265/1‑139 (MQ=255)                                                                   tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   >  2:393997/1‑139 (MQ=255)                                                                      cccGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGc                                                                <  1:156952/139‑1 (MQ=255)                                                                      cccGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGc                                                                <  1:393997/139‑1 (MQ=255)                                                                           tcggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCa                                                           <  1:77718/139‑1 (MQ=255)                                                                           tcggtGTTAAGGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCa                                                           <  2:38292/139‑1 (MQ=255)                                                                            cggtGTTAATGCCAAGCAGCCTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGAAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  2:42719/139‑1 (MQ=255)                                                                                     tGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTaa                                                  >  1:400921/1‑138 (MQ=255)                                                                                         aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCa                                                           >  1:78071/1‑125 (MQ=255)                                                                                         aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCa                                                           <  2:78071/125‑1 (MQ=255)                                                                                         aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATagag                                             >  1:91628/1‑139 (MQ=255)                                                                                         aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATagag                                             >  1:207318/1‑139 (MQ=255)                                                                                                         ttCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGc                             >  1:267783/1‑139 (MQ=255)                                                                                                            aGATCTCCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTACCATTCCAGATCCCCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTa                           <  1:17720/138‑1 (MQ=255)                                                                                                            aGATCTCCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTa                           <  2:287995/138‑1 (MQ=255)                                                                                                                 ttCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAg                                                                                                                        <  2:269389/40‑1 (MQ=255)                                                                                                                 ttCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAg                                                                                                                        >  1:269389/1‑40 (MQ=255)                                                                                                                   ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCccac                   <  2:352849/139‑1 (MQ=255)                                                                                                                           gCACGACGCGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACt           <  2:285901/139‑1 (MQ=255)                                                                                                                           gCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACt           <  2:342973/139‑1 (MQ=255)                                                                                                                           gCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACt           <  1:247265/139‑1 (MQ=255)                                                                                                                                    gcgcgGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTaa  <  1:110394/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAA  >  NZ_CP009273/3589085‑3589354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑