Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,836,431	A→G	V240A (GTC→GCC)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,836,431	0	A	G	100.0%	157.7 / NA	42	V240A (GTC→GCC)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/19);  total (23/19)

CCATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGACGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTTA  >  NZ_CP009273/3836296‑3836563                                                                                                                                        |                                                                                                                                     ccATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    <  1:392558/138‑1 (MQ=255)         tAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAata                                                                                                                           >  2:192971/1‑139 (MQ=255)              ccGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAgg                                                                                                                      <  2:53648/139‑1 (MQ=255)                gCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCg                                                                                                                    >  2:141542/1‑139 (MQ=255)                gCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCg                                                                                                                    >  2:394747/1‑139 (MQ=255)                gCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCg                                                                                                                    >  1:355242/1‑139 (MQ=255)                                  aTTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAcgccgc                                                                                                  >  1:438701/1‑139 (MQ=255)                                           ccTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGaa                                                                                         >  2:236350/1‑139 (MQ=255)                                            cTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGaa                                                                                         >  1:417225/1‑138 (MQ=255)                                              gatgaATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACAt                                                                                      >  1:90737/1‑139 (MQ=255)                                              gatgaATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACAt                                                                                      >  2:70858/1‑139 (MQ=255)                                                            gTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAaataat                                                                        >  1:177976/1‑139 (MQ=255)                                                             ttGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATc                                                                       <  2:417225/139‑1 (MQ=255)                                                             ttGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATc                                                                       <  1:70858/139‑1 (MQ=255)                                                                aTCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCca                                                                     >  2:71267/1‑138 (MQ=255)                                                                         aaGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGAc                                                            <  2:177976/138‑1 (MQ=255)                                                                          aGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGt                                                          <  2:141718/139‑1 (MQ=255)                                                                          aGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGt                                                          <  2:90737/139‑1 (MQ=255)                                                                          aGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGt                                                          >  1:274833/1‑139 (MQ=255)                                                                             agaAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       <  1:290541/139‑1 (MQ=255)                                                                               aacaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    >  1:440111/4‑60 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    <  2:440111/60‑1 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGTAAGACGTcacca                                                     <  2:438701/139‑1 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       >  1:142831/1‑137 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       <  2:142831/137‑1 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       <  1:300003/137‑1 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       >  2:300003/1‑137 (MQ=255)                                                                               aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCACCAGCAAGACGTcacc                                                      >  2:298488/1‑138 (MQ=255)                                                                                  aTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACaa                                                  <  1:429235/139‑1 (MQ=255)                                                                                             gAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAg                                        >  1:97301/1‑138 (MQ=255)                                                                                                          aaCGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGa                           >  2:278687/1‑138 (MQ=255)                                                                                                          aaCGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGa                           >  2:302607/1‑138 (MQ=255)                                                                                                          aaCGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGa                           >  1:1720/1‑138 (MQ=255)                                                                                                             gcgcCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAAt                       <  2:132328/139‑1 (MQ=255)                                                                                                                ccgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGAc                    <  1:394747/139‑1 (MQ=255)                                                                                                                  gcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGt                  <  2:49155/139‑1 (MQ=255)                                                                                                                             cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGacaac         <  2:197716/137‑1 (MQ=255)                                                                                                                             cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGacaac         <  1:71267/137‑1 (MQ=255)                                                                                                                             cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGacaac         <  1:119416/137‑1 (MQ=255)                                                                                                                             cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAacac       >  2:293976/1‑139 (MQ=255)                                                                                                                             cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAacac       >  1:100963/1‑139 (MQ=255)                                                                                                                                  ccAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTta  >  1:207768/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     CCATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGACGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTTA  >  NZ_CP009273/3836296‑3836563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑