Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,841,593	A→G	F131S (TTC→TCC)	uhpC ←	MFS transporter family glucose‑6‑phosphate receptor UhpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,841,593	0	A	G	100.0%	143.5 / NA	40	F131S (TTC→TCC)	uhpC	MFS transporter family glucose‑6‑phosphate receptor UhpC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/17);  total (23/17)

CCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGCTGACAA  >  NZ_CP009273/3841462‑3841725                                                                                                                                    |                                                                                                                                     ccATCACAAGGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACGCCGGTGAACCCCAGCCCTGGGAAAAg                                                                                                                                 <  1:246438/137‑1 (MQ=255)      acaATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCa                                                                                                                          <  1:327443/139‑1 (MQ=255)                tGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCagag                                                                                                                >  2:177483/1‑139 (MQ=255)                      cgccgcCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACgg                                                                                                           >  2:159714/1‑138 (MQ=255)                         cgccgACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCaaa                                                                                                       <  2:16747/139‑1 (MQ=255)                                        ccGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGt                                                                                        >  1:337760/1‑139 (MQ=255)                                             ttCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgag                                                                                    <  1:322628/138‑1 (MQ=255)                                                     tGCCCCCCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGcc                                                                               <  2:162138/135‑1 (MQ=255)                                                     tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGcc                                                                               >  1:162138/1‑135 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCaaa                                                                                                       >  1:190798/1‑106 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCaaa                                                                                                       <  2:190798/106‑1 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                      >  1:139616/1‑139 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                      >  1:362584/1‑139 (MQ=255)                                                                          ggTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTg                                                      <  1:189882/139‑1 (MQ=255)                                                                          ggTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTg                                                      <  1:159714/139‑1 (MQ=255)                                                                                      ccAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGt                                                                                                                             >  2:19725/1‑56 (MQ=255)                                                                                      ccAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGt                                                                                                                             <  1:19725/56‑1 (MQ=255)                                                                                      ccAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGccc                                           >  1:150173/1‑138 (MQ=255)                                                                                      ccAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAACCCAAACAGAATGTTGATAATGCCCGTGTCGATAAGccc                                           >  1:197164/1‑138 (MQ=255)                                                                                        aGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTAt                                        <  2:337760/139‑1 (MQ=255)                                                                                              ttAACAGACGCGTACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCa                                   >  1:297214/1‑138 (MQ=255)                                                                                              ttAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACa                                                                          <  2:344530/99‑1 (MQ=255)                                                                                              ttAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACa                                                                          >  1:344530/1‑99 (MQ=255)                                                                                                    gACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAAt                             >  1:49303/1‑138 (MQ=255)                                                                                                     aCGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATaa                           >  1:93438/1‑139 (MQ=255)                                                                                                               acCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCt                                          >  2:58436/1‑114 (MQ=255)                                                                                                               acCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCt                                          <  1:58436/114‑1 (MQ=255)                                                                                                                 cGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAg               >  2:246367/1‑139 (MQ=255)                                                                                                                 cGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAg               <  1:144269/139‑1 (MQ=255)                                                                                                                   gTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACggg                       <  2:206938/129‑1 (MQ=255)                                                                                                                   gTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACggg                       >  1:206938/1‑129 (MQ=255)                                                                                                                   gTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGc              >  2:30676/1‑138 (MQ=255)                                                                                                                      aaCCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATc          <  1:246367/139‑1 (MQ=255)                                                                                                                       aCCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCa                                                                                                         >  1:5579/1‑43 (MQ=255)                                                                                                                       aCCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCa                                                                                                         <  2:5579/43‑1 (MQ=255)                                                                                                                       aCCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCg         >  2:73664/1‑139 (MQ=255)                                                                                                                        ccccAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGc        >  1:219171/1‑139 (MQ=255)                                                                                                                           cAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAAc                                                                           >  1:33622/1‑69 (MQ=255)                                                                                                                           cAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAAc                                                                           <  2:33622/69‑1 (MQ=255)                                                                                                                              cccTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGCTGACaa  >  1:255537/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                     CCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGCTGACAA  >  NZ_CP009273/3841462‑3841725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑