Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,853,103	A→G	F125S (TTC→TCC)	yidK ←	solute:sodium symporter family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,853,103	0	A	G	100.0%	86.9 / NA	23	F125S (TTC→TCC)	yidK	solute:sodium symporter family transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/10);  total (13/10)

AGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCT  >  NZ_CP009273/3852968‑3853231                                                                                                                                        |                                                                                                                                 aGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAg                                                                                                                                >  2:387293/1‑138 (MQ=255)       aTTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGt                                                                                                                         <  1:401231/139‑1 (MQ=255)                aGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGataa                                                                                                                >  2:392401/1‑139 (MQ=255)                     gATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgc                                                                                                           <  2:1909/139‑1 (MQ=255)                      aTAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgcg                                                                                                          >  1:182125/1‑139 (MQ=255)                                  tGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCa                                                                                               >  1:386997/1‑138 (MQ=255)                                            gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgcg                                                                                                          >  1:121508/1‑117 (MQ=255)                                            gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgcg                                                                                                          <  2:121508/117‑1 (MQ=255)                                            gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                    >  2:301032/1‑139 (MQ=255)                                                           cGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGAt                                                                     >  2:223921/1‑139 (MQ=255)                                                                     gCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATg                                                           >  1:282484/1‑139 (MQ=255)                                                                         ttGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                         <  2:360542/137‑1 (MQ=255)                                                                                 cAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGa                                               <  1:207998/139‑1 (MQ=255)                                                                                   aCGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGa                                               >  2:207998/1‑137 (MQ=255)                                                                                        ccGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGcgcg                                        <  2:182125/139‑1 (MQ=255)                                                                                                     cAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAg                           >  2:19734/1‑139 (MQ=255)                                                                                                        tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcgc                        >  2:288156/1‑139 (MQ=255)                                                                                                        tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcgc                        >  1:88204/1‑139 (MQ=255)                                                                                                          ggCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGcgcg                       <  1:370512/138‑1 (MQ=255)                                                                                                                       cGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGt         <  1:223921/139‑1 (MQ=255)                                                                                                                             tGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                          <  2:244953/100‑1 (MQ=255)                                                                                                                             tGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                          >  1:244953/1‑100 (MQ=255)                                                                                                                               gCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCt  <  2:386997/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 AGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCT  >  NZ_CP009273/3852968‑3853231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑